Electrochemistry of Freely Diffusing Mediators in Polyelectrolyte Membranes Used for Blood Glucose Test Strips with a High Upper Limit of the Linear Range

Engineering Proceedings

article

Vita Nikitina¹  Ivan Solovyev²  Arkady Karyakin¹ 

[1] Chemistry Faculty, M.V. Lomonosov Moscow State University;Materials Science Faculty, M.V. Lomonosov Moscow State University

关键词: glucose;    test strip;    biosensor;    mediator;    diffusion;    membrane;    polymer;    chitosan;   

DOI  :  10.3390/IECB2023-14603

来源: mdpi

【 摘 要 】

Co-immobilization of low-molecular-weight mediators and glucose oxidase in polyelectrolyte membranes results in glucose test strips operating in the millimolar concentration range. The density and charge of polyelectrolyte membranes formed on the surface of the screen-printed electrodes allow the diffusion of mediators to be controlled. Negatively charged perfluorosulfonated ionomer (PFSI) hampers the diffusion of the commonly used ferricyanide (III) ion, while the hexammine ruthenium (III) cation apparent diffusion coefficient in PFSI membrane remains the same as without the membrane. In contrast to PFSI, electrode modification with positively charged chitosan leads to additional adsorption of potassium hexacyanoferrate on the membrane. Additionally, the rate of mediator leakage from the membrane was found to govern the sensitivity of the resulting biosensors. The leakage rate also depends on the density and charge of the polyelectrolyte and mediator. However, the main advantage of the proposed simple approach of single-step deposition of three-component membrane-forming mixtures on the screen-printed electrodes is the extended upper limit of the linearity: 30–50 mM glucose. Hence, the obtained test strips are suitable for glucose detection in undiluted blood.

【 授权许可】

Unknown