Effects of Electric Potential Treatment of a Chromium Hexacyanoferrate Modified Biosensor Based on PQQ-Dependent Glucose Dehydrogenase

Sensors

Ta-Feng Tseng¹  Yang-Li Yang¹  Yuh-Jiuan Lin² 

[1] Biomedical Engineering Department, Chung-Yuan Christian University, No. 200, Zhongbei Rd., Zhongli City, Taoyuan County 32023, Taiwan; E-Mails:;Medical Electronics and Device Technology Center, Industrial Technology Research Institute, Rm. 334, Bldg. 53, 195, Sec. 4, Chung Hsing Rd., Chutung, Hsinchu County 31040, Taiwan; E-Mail:

关键词: biosensor;    chromium hexacyanoferrate;    PQQ-dependent glucose dehydrogenase;    mediator;    potential treatment;   

DOI  :  10.3390/s100706347

来源: mdpi

【 摘 要 】

A novel potential treatment technique applied to a glucose biosensor that is based on pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) and chromium hexacyanoferrate (CrHCF) incorporated into a platinum (Pt) electrode was demonstrated. CrHCF, serving as a mediator, was electrochemically deposited on the Pt electrode as ascertained by CV, SEM, FTIR and XPS measurements. The potential treatment of CrHCF, which converts Fe(II) to Fe(III), enables the glucose detection. The amperometric measurement linearity of the biosensor was up to 20 mM (R = 0.9923), and the detection sensitivity was 199.94 nA/mM per cm². More importantly, this biosensor remained stable for >270 days.

【 授权许可】

CC BY   
© 2010 by the authors licensee MDPI, Basel, Switzerland.