Jundishapur Journal of Natural Pharmaceutical Products | |
Antiparasitic Effects and Cellular Mechanisms of Formononetin (a Natural Isoflavone) Against Hydatid Cyst Protoscoleces | |
article | |
Setareh Dastyar Haghighi1  Hossein Mahmoudvand1  Amal Khudair Khalaf2  Ahmad Adineh3  Ali Mohammad Maleki4  Javad Ghasemian Yadegari1  | |
[1] Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences;Department of Microbiology, College of Medicine, University of Thiqar;Department of Toxicology, Faculty of Pharmacy, Lorestan University of Medical Sciences;Department of Pharmacotherapy, Faculty of Pharmacy, Lorestan University of Medical Sciences | |
关键词: Echinococcus granulosus; Scolicidal; Apoptosis; Plasma Membrane; In Vitro and Ex Vivo; | |
DOI : 10.5812/jjnpp-129302 | |
学科分类:药理学 | |
来源: Jundishapur Journal of Natural Pharmaceutical Products | |
【 摘 要 】
Background: The chemical agents applied to reduce the complications of hydatid cyst surgery are not free of side effects. Formononetin (FMN), as a natural isoflavone, has been shown to have various therapeutic benefits. Objectives: We studied the in vitro and ex vivo protoscolicidal activity and cellular mechanisms of FMN against hydatid cyst protoscoleces. Methods: Formononetin at 75, 150, and 300 μg/mL was mixed with hydatid cyst protoscoleces (103/mL), and the viability was determined in 5 to 60 min through eosin staining assay. The effect of FMN on caspase-3 activity was tested through a commercial colorimetric protease kit. In addition, the penetrability of the plasma membrane of protoscoleces after exposure to FMN was also determined by the SYTOX assay. Results: Formononetin at 300 μg/mL completely destroyed hydatid cyst protoscoleces after 30 minutes. While at 150 μg/mL, after 60 minutes of contact, 100% of protoscoleces were eliminated. By ex vivo assay, FMN showed its antiparasitic effect for longer periods. Based on these results, FMN at 300 μg/mL concentration completely eliminated hydatid cyst protoscoleces after 60 minutes of exposure. While at a concentration of 150 μg/mL after 60 minutes, 89.6% of protoscoleces were destroyed. Formononetin noticeably increased (P < 0.001) the activity of caspase-3 and the permeability of protoscoleces dose-dependently. Conclusions: Formononetin as a natural product showed promising effects on the protoscoleces of hydatid cysts, indicating that it can be considered a valuable scolicidal agent. However, additional investigations are necessary to evaluate its efficacy in animal models and human subjects.
【 授权许可】
CC BY-NC
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