Nanostructured lipid carriers of ivermectin as a novel drug delivery system in hydatidosis

Parasites & Vectors

Peyman Heydarian¹  Roghayeh Norouzi²  Hamed Hamishehkar³  Saba Rajabi³  Ehsan Ahmadpour³  Zahra Godrati-Azar³  Maryam Mohammadi⁴  Sanam Nami⁵  Adel Spotin⁶  Gregorio Perez-Cordon⁷ 

[1] Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences;Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz;Drug Applied Research Center, Tabriz University of Medical Sciences;Food and Drug Safety Research Center, Tabriz University of Medical Sciences;Immunology Research Center, Tabriz University of Medical Sciences;Infectious and Tropical Disease Research Center, Tabriz University of Medical Sciences;National Cryptosporidium Reference Unit, Public Health Wales Microbiology, Singleton Hospital;

关键词: Hydatid cyst;    Ivermectin;    Scolicidal;    Treatment;    Nano lipid carriers;    Apoptosis;   

DOI  :  10.1186/s13071-019-3719-x

来源: DOAJ

【 摘 要 】

Abstract Background The larval stage of the tapeworm Echinococcus granulosus is the causative agent of hydatid disease in humans. This zoonotic parasitic infection remains a major health problem in certain areas of the world where is still endemic. In view of the ineffectiveness of some drug treatments, the surgical removal of cysts remains the preferred treatment option together with the administration of albendazole and mebendazole. However, severe side effects of these drugs have been reported which demands developing new scolicidal agents that confer suitable efficacy and fewer side effects during surgery. Methods To that purpose, in the present work we assessed the effectiveness of ivermectin (IVM), a macrocyclic lactone endectocide that has shown to be an effective nematocidal drug against other important parasitic infections. To overcome the limitations observed in some drug formulations and resistance, we used nano lipid carriers (NLCs) as a targeted and sustained drug delivery system for IVM. We evaluated the in vitro cestocidal and apoptotic effects of NLCs-loaded IVM versus IVM by quantifying the expression of caspase-3 mRNA. Results We found that after 60 and 120 min of administration, 800 μg/ml and 400 μg/ml NLCs-loaded IVM induced 100% mortality, respectively. On the other hand, the 800 μg/ml of IVM induced 100% mortality rate 150 min after administration. Additionally, we found that NLCs-loaded IVM induced higher mRNA caspase-3 expression suggesting a more potent apoptotic effect on the parasite. Conclusions These data suggest that NLCs-loaded IVM may be a promising alternative to current treatments although in vivo studies are needed.

【 授权许可】

Unknown