Callus-mediated Somatic Embryogenesis and Plant Regeneration in Vanda tricolor Lindl. var. Pallida

Jordan Journal of Biological Sciences

article

Popy Hartatie Hardjo¹  Wina Dian Savitri¹  Ida Bagus Made Artadana¹  Sulistyo Emantoko Dwi Putra¹  Elizabeth Pio Parac²  Asad Jan³ 

[1] Faculty of Biotechnology, University of Surabaya;College of Agriculture and Agri-Industries, Caraga State University;Institute of Biotechnology and Genetic Engineering, University of Agriculture

关键词: Embryogenic calli;    Histology;    Half-strength Murashige-Skoog medium;    Micropropagation;    Orchid;    Pro-embryo;   

DOI  :  10.54319/jjbs/140508

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

In this study, a protocol to induce indirect somatic embryogenesis from the basal leaf segments of Vanda tricolor Lindl. var. Pallida has been developed. 20TThe experiments consisted of two stages، i.e. induction of SEs from calli and regeneration of –1 –1plantlet from SEs. 20TEmbryogenic calli obtained from previous experiment (0.05 mg LP P NAA + 0.01 mg LP P BAP) were used –1 to induce somatic embryos (SEs) on half-strength Murashige and Skoog (MS) medium supplemented with (0.05 mg LP PtoP P –1 –1 0.20 mg LP ) 6P -benzylaminopurine (BAP) alone or in combination with 0.01 mg LP Pα-naphtalene acetic acidP P(NAA). –1 –1 Embryogenic calli, those cultured on 0.05 mg LP P BAP and 0.01 mg LP PNAA resulted in 90 % induction of SE structures at 30 d of culture period. Histological observation exhibited development of pro-embryo to form completed embryo. The pattern of SEs development started from embryogenic callus to form pro-embryo, followed by globular phase at 10 d of culture. Globular embryo elongated to form suspensor at 20 d of incubation period, and completed embryo. Regeneration of SEs into complete plantlets was attained on the half-strength MS medium without addition of any plant growth regulator (PGR). Based on the results of the present study, it can be concluded that 20Thalf-strength MS medium supplemented with –1 –120TNAA 0.01 mg LP RP Rand BAP 0.05 mg LP P is the best medium for induction of SEs from embryogenic calli.

【 授权许可】

CC BY