Multiplicación in vitro y embriogénesis somática de Perezia pinnatifida (Asteraceae) planta medicinal andina

Revista Peruana de Biologia

Espinoza del Río, Rosa¹  Olivera-Gonzales, Percy¹  Tamariz-Angeles, Carmen¹ 

[1] Universidad Nacional Santiago Antúnez de Mayolo, Huaraz, Perú

关键词: Planta medicinal;    Micropropagación;    embrión somático;    Cultivo de tejidos vegetales;    propagación clonal;    Medicinal plant;    Micropropagation;    Somatic embryo;    Plant tissue culture;    clonal propagation.;   

DOI  :  10.15381/rpb.v24i3.13911

学科分类：生物科学（综合）

来源: Universidad Nacional Mayor de San Marcos * Facultad de Ciencias Biologicas

【 摘 要 】

This work inform on in vitro propagation of the "valeriana" Perezia pinnatifida (Humb. & Bonpl.) Wedd. Shoot multiplication and indirect somatic embryogenesis methodologies were performed. The basal culture medium for all stages was Murashige and Skoog, middle of salts supplemented with 2.0% sucrose, 0.3% phytagel and pH 5.67; the treatments were prepared with or without phytohormones. The hormonal supplements for the shoot multiplication were: BAP 1.0 mg.L-1 + ANA 0.01 mg.L-1, and BAP 1.0 mg.L-1; for embryogenic callus induction were: ANA or 2,4-D (1.0 mg.L-1 and 2.0 mg.L-1); and for the embryo germination were: BAP (0.5 and 1.0 mg.L-1) or BAP 0.5 mg.L-1 + ANA 0.05 mg.L-1. BAP 1.0 mg.L-1 produced the higher number buds. For somatic embryogenesis, ANA 1.0 mg.L-1 induced a greater area of embryogenic callus, and BAP 0.5 mg.L-1 allowed major germination of the somatic embryos

【 授权许可】

CC BY-NC