Promoter activity and transcriptome analyses decipher functions of CgbHLH001 gene (Chenopodium glaucum L.) in response to abiotic stress

BMC Plant Biology

Research

Qinghui Yu¹  Juan Wang¹  Zixin Zhou²  Haiyan Lan² 

[1] Institute of Horticulture Crops, Xinjiang Academy of Agricultural Science, 830091, Urumqi, China;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 830017, Urumqi, China;

关键词: CgbHLH001;    Chenopodium glaucum;    Promoter activity;    Salt tolerance;    Transcriptome analysis;   

DOI  :  10.1186/s12870-023-04128-8

 received in 2022-08-23, accepted in 2023-02-15,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundOur previous studies revealed that CgbHLH001 transcription factor (TF) played an important role in abiotic stress tolerance, suggesting that its promoter was a potential target in response to stress signals. In addition, the regulatory mechanism of CgbHLH001 TF is still limited.ResultsIn the present study, a 1512 bp of 5’-flanking sequence of CgbHLH001 gene was identified, and the sequence carried quite a few of cis-acting elements. The gene promoter displayed strong activity and was induced by multiple abiotic stress. A series of 5’-deletions of the promoter sequence resulted in a gradual decrease in its activity, especially, the 5’ untranslated region (UTR) was necessary to drive promoter activity. Further, CgbHLH001 promoter drove its own gene overexpression ectopically at the transcriptional and translational levels, which in turn conferred the stress tolerance to transgenic Arabidopsis. Transcriptome analysis showed that salt stress induced a large number of genes involved in multiple biological regulatory processes. Differentially expressed genes (DEGs) that mediate phytohormone signal transduction and mitogen-activated protein kinase (MAPK) signaling pathway were widely induced and mostly upregulated under salt stress, and the transcription levels in PbHLH::bHLH-overexpressing transgenic lines were higher than that of 35S::bHLH overexpression.ConclusionsThe CgbHLH001 promoter exhibited a positive response to abiotic stress and its 5’ UTR sequence enhanced the regulation of gene expression to stress. A few important pathways and putative key genes involved in salt tolerance were identified, which can be used to elucidate the mechanism of salt tolerance and decipher the regulatory mechanism of promoters to develop an adaptation strategy for desert halophytes.

【 授权许可】

CC BY   
© The Author(s) 2023

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