The DNA replication‐related element (DRE)–DRE‐binding factor (DREF) system may be involved in the expression of the Drosophila melanogaster TBP gene

FEBS Letters

Han, Kyuhyung⁴  Yoon, Jaeseung²  Cho, Nam-Young³  Ryu, Yoonseok²  Yoo, Mi-Ae¹  Matsukage, Akio⁵  Baek, Kwanghee²  Oh, Younsang²  Choi, Tae-Yeong¹ 

[1] Department of Molecular Biology, College of Natural Science, Pusan National University, Pusan 609-735, South Korea;Department and Institute of Genetic Engineering, Kyung Hee University, Yongin City 449-701, South Korea;Department of Microbiology, Taejon University, Taejon 300-716, South Korea;Department of Genetic Engineering, Hallym University, Chunchon 200-702, South Korea;Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa-Ku, Nagoya 464, Japan

关键词: TATA box-binding protein;    DNA replication-related element;    Gene expression;    Promoter activity;    Drosophila melanogaster;    DRE;    DNA replication-related element;    DREF;    DRE-binding factor;    TBP;    TATA box binding protein;    GST;    glutathione S-transferase;   

DOI  :  10.1016/S0014-5793(00)02085-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The TATA box binding protein (TBP) is a general transcription factor required for initiation by all three eukaryotic RNA polymerases. Previously, we found that the promoter region of the Drosophila melanogaster TBP gene contains three sequences similar to the DNA replication-related element (DRE) (5′-TATCGATA). In the present study, we found that the DRE-like sequences are also present in the promoter of the Drosophila virilis TBP gene, suggesting a role for these sequences in TBP expression. Band mobility shift assays revealed that oligonucleotides containing sequences similar to the DRE of D. melanogaster TBP gene promoter form specific complexes with a factor in a Kc cell nuclear extract and with recombinant DRE-binding factor (DREF). Furthermore, these complexes were either supershifted or diminished by monoclonal antibodies to DREF. Transient luciferase assays demonstrated that induction of mutations in two DRE-related sequences at positions −223 and −63 resulted in an extensive reduction of promoter activity. Thus, the DRE–DREF system appears to be involved in the expression of the D. melanogaster TBP gene.

【 授权许可】

Unknown