IAPP/amylin gene transcriptional control region: evidence for negative regulation

FEBS Letters

Lips, C.J.M.²  Jansz, H.S.¹  Soeller, W.³  Mosselman, S.¹  de Wit, L.¹  Höppener, J.W.M.¹ 

[1] Institute of Molecular Biology and Medical Biotechnology, Padualaan 8, 3584 CH Utrecht, The Netherlands;Laboratory of Physiological Chemistry, Department of Internal Medicine, University of Utrecht, The Netherlands;Pfizer Central Research, Department of Molecular Genetics, Groton, CT 06340, USA

关键词: Human IAPP/amylin gene;    Promoter activity;    Negative regulation;    Pancreatic β-cell;    Diabetes mellitus type 2;   

DOI  :  10.1016/0014-5793(90)80365-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Aberrant expression of the islet amyloid polypeptide (IAPP) gene might be involved in the pathogenesis of nun-insulin-dependent diabetes mellitus (NIDDM). Here, we report that IAPP promoter-luciferase constructs revealed tissue-specific activity. This activity was not mediated by cAMP. Sequential 5' deletions of the IAPP promoter caused a progressive derepression of the IAPP gene promoter in IAPP-producing cells. Comparison of the nucleotide sequence of the IAPP promoter with that of the insulin promoter (both active in pancreatic β-cells) reveals two sequence elements of putative importance: an insulin enhancer-like sequence and an element which corresponds to a protected domain in rat insulin I gene promoter footprint experiments.

【 授权许可】

Unknown