Two regions in c‐myb proto‐oncogene product negatively regulating its DNA‐binding activity

FEBS Letters

Tanaka, Yasunori¹  Ishii, Shunsuke¹  Nomura, Teruaki¹ 

[1] Laboratory of Molecular Genetics, Tsukuba Life Science Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan

关键词: myb proto-oncogene;    Functional domain;    DNA binding;    Negative regulation;    Protein–protein interaction;   

DOI  :  10.1016/S0014-5793(97)00900-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The c-myb proto-oncogeneproduct (c-Myb) is a transcriptional regulator that binds to the specific DNA sequence. Deletion of the negative regulatory domain (NRD) in the carboxyl-proximal region of c-Myb results in both increased trans-activating capacity and oncogenic activation. One possible mechanism to modulate c-Myb activity is a regulation of DNA-binding activity. However, it is not known whether any region in NRD affects the in vivo DNA-binding activity of c-Myb. Using the highly transfectable cell line 293T, we developed a system to precisely measure the DNA-binding activity of Myb expressed in mammalian cells. Using this system, two regions in NRD were shown to repress DNA-binding activity. These results suggest that DNA-binding activity of c-Myb is independently regulated by multiple mechanisms through these subdomains.

【 授权许可】

Unknown