Functional analysis of the global repressor Tup1 for maltose metabolism in Saccharomyces cerevisiae: different roles of the functional domains

Microbial Cell Factories

Research

Dong-Guang Xiao¹  Ai-Qun Yu¹  Li Pi¹  Xiao-Wen Bai¹  Cui-Ying Zhang¹  Xue Lin² 

[1] Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, 300457, Tianjin, People’s Republic of China;Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, 300457, Tianjin, People’s Republic of China;College of Food Science and Technology, Hainan University, 570228, Haikou, China;

关键词: Baker’s yeast;    Tup1;    Functional domain;    Maltose metabolism;    Glucose repression;   

DOI  :  10.1186/s12934-017-0806-6

 received in 2017-09-05, accepted in 2017-10-31,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTup1 is a general transcriptional repressor of diverse gene families coordinately controlled by glucose repression, mating type, and other mechanisms in Saccharomyces cerevisiae. Several functional domains of Tup1 have been identified, each of which has differing effects on transcriptional repression. In this study, we aim to investigate the role of Tup1 and its domains in maltose metabolism of industrial baker’s yeast. To this end, a battery of in-frame truncations in the TUP1 gene coding region were performed in the industrial baker’s yeasts with different genetic background, and the maltose metabolism, leavening ability, MAL gene expression levels, and growth characteristics were investigated.ResultsThe results suggest that the TUP1 gene is essential to maltose metabolism in industrial baker’s yeast. Importantly, different domains of Tup1 play different roles in glucose repression and maltose metabolism of industrial baker’s yeast cells. The Ssn6 interaction, N-terminal repression and C-terminal repression domains might play roles in the regulation of MAL transcription by Tup1 for maltose metabolism of baker’s yeast. The WD region lacking the first repeat could influence the regulation of maltose metabolism directly, rather than indirectly through glucose repression.ConclusionsThese findings lay a foundation for the optimization of industrial baker’s yeast strains for accelerated maltose metabolism and facilitate future research on glucose repression in other sugar metabolism.

【 授权许可】

CC BY   
© The Author(s) 2017