YULINK regulates vascular formation in zebrafish and HUVECs

Biological Research

Research Article

Hsin-Hung Lin¹  Ming-Wei Kuo²  Tan-Chi Fan²  Alice L. Yu³  John Yu⁴ 

[1] Chemical Biology and Molecular Biophysics Program, International Graduate Program, Academia Sinica, Taipei, Taiwan;Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan;Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan;Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan;Department of Pediatrics, University of California, San Diego, CA, USA;Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou, 333, Taoyuan, Taiwan;Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan;

关键词: YULINK;    Vasculogenesis;    Endocytosis;    Clathrin;    VEGFR2;    VEGF;    RHOB;    EPS15;    RAB33B;    TICAM2;   

DOI  :  10.1186/s40659-023-00415-8

 received in 2022-08-24, accepted in 2023-01-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe distinct arterial and venous cell fates are dictated by a combination of various genetic factors which form diverse types of blood vessels such as arteries, veins, and capillaries. We report here that YULINK protein is involved in vasculogenesis, especially venous formation.MethodsIn this manuscript, we employed gene knockdown, yeast two-hybrid, FLIM-FRET, immunoprecipitation, and various imaging technologies to investigate the role of YULINK gene in zebrafish and human umbilical vein endothelial cells (HUVECs).ResultsKnockdown of YULINK during the arterial-venous developmental stage of zebrafish embryos led to the defective venous formation and abnormal vascular plexus formation. Knockdown of YULINK in HUVECs impaired their ability to undergo cell migration and differentiation into a capillary-like tube formation. In addition, the phosphorylated EPHB4 was decreased in YULINK knockdown HUVECs. Yeast two-hybrid, FLIM-FRET, immunoprecipitation, as well as imaging technologies showed that YULINK colocalized with endosome related proteins (EPS15, RAB33B or TICAM2) and markers (Clathrin and RHOB). VEGF-induced VEGFR2 internalization was also compromised in YULINK knockdown HUVECs, demonstrating to the involvement of YULINK.ConclusionThis study suggests that YULINK regulates vasculogenesis, possibly through endocytosis in zebrafish and HUVECs.

【 授权许可】

CC BY   
© The Author(s) 2023

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