YULINK regulates vascular formation in zebrafish and HUVECs

Biological Research

article

Lin, Hsin-Hung¹  Kuo, Ming-Wei²  Fan, Tan-Chi²  Yu, Alice L.²  Yu, John² 

[1] Chemical Biology and Molecular Biophysics Program, International Graduate Program;Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital at Linkou;Department of Pediatrics, University of California;Institute of Cellular and Organismic Biology

关键词: YULINK;    Vasculogenesis;    Endocytosis;    Clathrin;    VEGFR2;    VEGF;    RHOB;    EPS15;    RAB33B;    TICAM2;   

DOI  :  10.1186/s40659-023-00415-8

学科分类：社会科学、人文和艺术（综合）

来源: BioMed Central

【 摘 要 】

The distinct arterial and venous cell fates are dictated by a combination of various genetic factors which form diverse types of blood vessels such as arteries, veins, and capillaries. We report here that YULINK protein is involved in vasculogenesis, especially venous formation. In this manuscript, we employed gene knockdown, yeast two-hybrid, FLIM-FRET, immunoprecipitation, and various imaging technologies to investigate the role of YULINK gene in zebrafish and human umbilical vein endothelial cells (HUVECs). Knockdown of YULINK during the arterial-venous developmental stage of zebrafish embryos led to the defective venous formation and abnormal vascular plexus formation. Knockdown of YULINK in HUVECs impaired their ability to undergo cell migration and differentiation into a capillary-like tube formation. In addition, the phosphorylated EPHB4 was decreased in YULINK knockdown HUVECs. Yeast two-hybrid, FLIM-FRET, immunoprecipitation, as well as imaging technologies showed that YULINK colocalized with endosome related proteins (EPS15, RAB33B or TICAM2) and markers (Clathrin and RHOB). VEGF-induced VEGFR2 internalization was also compromised in YULINK knockdown HUVECs, demonstrating to the involvement of YULINK. This study suggests that YULINK regulates vasculogenesis, possibly through endocytosis in zebrafish and HUVECs.

【 授权许可】

CC BY|CC0