Integrative metabolomics of plasma and PBMCs identifies distinctive metabolic signatures in Behçet’s disease

Arthritis Research & Therapy

Research

Soo Jin Park¹  Do Yup Lee²  Mi Jin Park³  Eun-So Lee³  Sun Park⁴ 

[1] Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea;Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea;Center for Food and Bioconvergence, Research Institute for Agricultural and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, 08826, Seoul, Republic of Korea;Department of Dermatology, Ajou University School of Medicine, 164 Worldcup-ro, Yeongtong-gu, 16499, Suwon, Republic of Korea;Department of Microbiology, Ajou University School of Medicine, 16499, Suwon, Republic of Korea;

关键词: Metabolomics;    Lipidomics;    Behçet’s disease;    Autoimmune disease;   

DOI  :  10.1186/s13075-022-02986-5

 received in 2022-08-13, accepted in 2022-12-26,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundBehçet’s disease (BD) is a systemic inflammatory disease that involves various organs. The clinical manifestation-based diagnosis of BD is a time-consuming process, which makes it difficult to distinguish from patients with similar symptoms. Moreover, an authentic biomarker has not been developed for accurate diagnosis yet. Our current study investigated the unique metabolic signatures of BD and explored biomarkers for precise diagnosis based on an untargeted metabolomic approach.MethodsIntegrative metabolomic and lipidomic profiling was performed on plasma samples of BD patients (n = 40), healthy controls (HCs, n = 18), and disease controls (DCs, n = 17) using GC-TOF MS and LC-Orbitrap MS. Additionally, the lipid profiles of 66 peripheral blood mononuclear cells (PBMCs) were analyzed from 29 BD patients, 18 HCs, and 19 DCs.ResultsPlasma metabolic dysfunction in BD was determined in carbohydrate, hydroxy fatty acid, and polyunsaturated fatty acid metabolisms. A plasma biomarker panel with 13 compounds was constructed, which simultaneously distinguished BD from HC and DC (AUCs ranged from 0.810 to 0.966). Dysregulated PBMC metabolome was signatured by a significant elevation in lysophosphatidylcholines (LPCs) and ether-linked lysophosphatidylethanolamines (EtherLPEs). Ten PBMC-derived lipid composites showed good discrimination power (AUCs ranged from 0.900 to 0.973). Correlation analysis revealed a potential association between disease activity and the metabolites of plasma and PBMC, including sphingosine-1 phosphate and EtherLPE 18:2.ConclusionsWe identified metabolic biomarkers from plasma PBMC, which selectively discriminated BD from healthy control and patients with similar symptoms (recurrent mouth ulcers with/without genital ulcers). The strong correlation was determined between the BD activity and the lipid molecules. These findings may lead to the development for diagnostic and prognostic biomarkers based on a better understanding of the BD pathomechanism.

【 授权许可】

CC BY   
© The Author(s) 2023

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