Aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4

Cancer Cell International

Research

Qing You¹  Hao Lu²  Haiyang Zhou²  Jian Zhang²  Zhiguo Wang²  Junnan Chen²  Jun Ying² 

[1] Department of Gastrointestinal Surgery, Second Affiliated Hospital of Naval Medical University, Shanghai, China;Division of Colorectal Surgery, Department of Surgery, Second Affiliated Hospital of Naval Medical University, 415 Fengyang Road, 200003, Shanghai, China;

关键词: Aspirin;    Colorectal cancer;    Chemotherapy resistance;    Toll-like receptor 4;   

DOI  :  10.1186/s12935-023-02847-4

 received in 2022-06-20, accepted in 2023-01-02,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundChemotherapy resistance is an important bottleneck affecting the efficacy of chemotherapy in colon cancer. Therefore, improving the chemotherapy sensitivity of colorectal cancer cells is of great significance for improving the prognosis of patients with colon cancer.MethodsCCK-8 assay was employed to examine the cell viability of colorectal cancer cell lines. Realtime-PCR and western blot were used to explore toll-like receptor 4 (TLR4) expression in colorectal cancer cell lines. The functions of TLR4 in the stemness of the colorectal cancer cell lines were analyzed by infecting cells with lentivirus containing TLR4 siRNA.ResultsWe found that aspirin could effectively enhance the chemosensitivity of CT26 and HCT116 colorectal cancer cell lines. Aspirin can also inhibit the stemness of colorectal cancer cell including inhibiting the number of clone formation and reducing the volume and number of cell spheres and inducing the down-regulation of stemness-related genes. Besides that, aspirin also lead to down-regulation of TLR4 expression in colorectal cancer cells. The TLR4 positive colorectal cancer cells demonstrated a higher chemotherapy resistance potential than TLR4 negative colorectal cancer cells. In addition, the stemness of TLR4 positive colorectal cancer cells is stronger than TLR4 negative colorectal cancer cells.ConclusionThe results of our study indicate that aspirin increases chemosensitivity of colorectal cancer cells and inhibits the expression of toll-like receptor 4.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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