Effects of sustained hyperprolactinemia in late gestation on the mammary parenchymal tissue transcriptome of gilts

BMC Genomics

Research Article

Marie-France Palin¹  Chantal Farmer¹  Anouk Caron² 

[1] Agriculture and Agri-Food Canada, Sherbrooke R & D Centre, Sherbrooke, QC, Canada;Université Laval, Québec, QC, Canada;

关键词: Domperidone;    Gestation;    Gilt;    Mammary gland;    Prolactin;    Transcriptomic;   

DOI  :  10.1186/s12864-023-09136-4

 received in 2022-07-22, accepted in 2023-01-13,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundGilts experiencing sustained hyperprolactinemia from d 90 to 109 of gestation showed an early onset of lactogenesis coupled with premature mammary involution. To better understand the molecular mechanisms underlying the premature mammary involution observed in these gilts, a transcriptomic analysis was undertaken. Therefore, this study aimed to explore the effect of hyperprolactinemia on the global transcriptome in the mammary tissue of late gestating gilts and identify the molecular pathways involved in triggering premature mammary involution.MethodsOn d 90 of gestation, gilts received daily injections of (1) canola oil until d 109 ± 1 of gestation (CTL, n = 18); (2) domperidone (to induce hyperprolactinemia) until d 96 ± 1 of gestation (T7, n = 17) or; (3) domperidone (until d 109 ± 1 of gestation (T20, n = 17). Mammary tissue was collected on d 110 of gestation and total RNA was isolated from six CTL and six T20 gilts for microarray analysis. The GeneChip® Porcine Gene 1.0 ST Array was used for hybridization. Functional enrichment analyses were performed to explore the biological significance of differentially expressed genes, using the DAVID bioinformatics resource.ResultsThe expression of 335 genes was up-regulated and that of 505 genes down-regulated in the mammary tissue of T20 vs CTL gilts. Biological process GO terms and KEGG pathways enriched in T20 vs CTL gilts reflected the concurrent premature lactogenesis and mammary involution. When looking at individual genes, it appears that mammary cells from T20 gilts can simultaneously upregulate the transcription of milk proteins such as WAP, CSN1S2 and LALBA, and genes triggering mammary involution such as STAT3, OSMR and IL6R. The down-regulation of PRLR expression and up-regulation of genes known to inactivate the JAK-STAT5 pathway (CISH, PTPN6) suggest the presence of a negative feedback loop trying to counteract the effects of hyperprolactinemia.ConclusionsGenes and pathways identified in this study suggest that sustained hyperprolactinemia during late-pregnancy, in the absence of suckling piglets, sends conflicting pro-survival and cell death signals to mammary epithelial cells. Reception of these signals results in a mammary gland that can simultaneously synthesize milk proteins and initiate mammary involution.

【 授权许可】

CC BY   
© Crown 2023

【 图 表 】

41116_2022_35_Article_IEq262.gif

41116_2022_35_Article_IEq259.gif

41116_2022_35_Article_IEq251.gif

41116_2022_35_Article_IEq244.gif

41116_2022_35_Article_IEq238.gif