Expression of IL-23 in gilt endometrium and oviduct after insemination with seminal plasma, spermatozoa or semen extender

BMC Research Notes

Caroline Fossum¹  Anna Svensson²  Anne-Marie Dalin²  Jatesada Jiwakanon³ 

[1] Department of Biomedical Science and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;Research Group for Animal Health Technology, Khon Kaen University, Khon Kaen, Thailand;

关键词: IL-23;    mRNA expression;    Gilt;    Endometrium;    Oviduct;    Insemination;   

DOI  :  10.1186/s13104-021-05630-8

来源: Springer

【 摘 要 】

ObjectiveInsemination with spermatozoa, seminal plasma and extender, cause a rapid inflammatory response in pig endometrium, characterized by an influx of neutrophils into the uterus. The transient inflammatory response to semen involves cytokine induction. Potential functions for Interleukin-23 (IL-23) in the inflammatory response to different insemination treatments were examined by studying mRNA expression and immunostaining in gilt oviduct and endometrium 35–40 h after insemination. Insemination was performed with seminal plasma (SP), spermatozoa (SPZ) without SP in the extender Beltsville thawing solution (BTS), or BTS alone. In control gilts an insemination catheter was inserted without anything being inseminated.ResultsResults showed that IL-23 mRNA was expressed in oviduct and endometrium after insemination regardless of treatment. There was an approximate two- to fourfold increase in expression of IL-23 mRNA in catheter-insertion control compared with SPZ, SP and BTS treatment groups. IL-23 immunolabelling was detected in a small number of separate cells and in the sub-epithelial connective tissue of the endometrium, the endosalpinx of isthmus and infundibulum.ConclusionIn conclusion, insemination with SP, SPZ in BTS, and BTS alone decreased the expression of IL-23 mRNA in the endometrium compared to catheter-insertion control, indicating a possible role for IL-23 in the inflammatory response after insemination in gilts.

【 授权许可】

CC BY