Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects

BMC Biology

Research Article

Haibin Yuan¹  Kenneth Wilson²  Kaiyu Liu³  Shigang Wu⁴  Ping Wang⁴  Hangwei Liu⁴  Swapan Chakrabarty⁴  Yutao Xiao⁴  Zhenxing Liu⁴  Wei Fan⁴  Bo Liu⁴  Chao Wu⁴  Weigang Zheng⁵  Conghui Liu⁶  Minghui Jin⁷  Yuan He⁷  Xiaokang Li⁸  Kongming Wu⁸ 

[1] College of Agronomy, Jilin Agricultural University, 130118, Changchun, China;Lancaster Environment Centre, Lancaster University, LAI 4YQ, Lancaster, UK;School of Life Sciences, Central China Normal University, 430079, Wuhan, China;Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China;Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China;College of Agronomy, Jilin Agricultural University, 130118, Changchun, China;Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China;Department of Clinical Oncology, University of Hong Kong, Hong Kong (Special Administrative Region), 999077, Hongkong, China;Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;

关键词: Genome assembly;    Comparative genomics;    Transcriptome;    Host adaptation;    Migration;    Cutworm;   

DOI  :  10.1186/s12915-022-01504-y

 received in 2022-06-20, accepted in 2022-12-16,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe black cutworm, Agrotis ipsilon, is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute to its widening distribution and increasing difficulty of control. However, knowledge about these traits is still limited.ResultsWe generated a high-quality chromosome-level assembly of A. ipsilon using PacBio and Hi-C technology with a contig N50 length of ~ 6.7 Mb. Comparative genomic and transcriptomic analyses showed that detoxification-associated gene families were highly expanded and induced after insects fed on specific host plants. Knockout of genes that encoded two induced ABC transporters using CRISPR/Cas9 significantly reduced larval growth rate, consistent with their contribution to host adaptation. A comparative transcriptomic analysis between tethered-flight moths and migrating moths showed expression changes in the circadian rhythm gene AiCry2 involved in sensing photoperiod variations and may receipt magnetic fields accompanied by MagR and in genes that regulate the juvenile hormone pathway and energy metabolism, all involved in migration processes.ConclusionsThis study provides valuable genomic resources for elucidating the mechanisms involved in moth migration and developing innovative control strategies.

【 授权许可】

CC BY   
© The Author(s) 2022

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