Vascular lipidomics analysis reveales increased levels of phosphocholine and lysophosphocholine in atherosclerotic mice

Nutrition & Metabolism

Research

Li Fan¹  Mi Tang¹  Shasha Xing¹  Xiao Yang¹  Haoyang Diao¹  Liling Xiong¹  Wenmei Guo¹  Erdan Luo¹  Li Zhang² 

[1] Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, 611731, Chengdu, China;Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, 611731, Chengdu, China;Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 200336, Shanghai, China;

关键词: Atherosclerosis;    Untargeted lipidomics;    ApoE;    Phosphocholines;    Lysophosphocholines;   

DOI  :  10.1186/s12986-022-00723-y

 received in 2022-10-04, accepted in 2022-12-30,  发布年份 2022

来源: Springer

【 摘 要 】

ObjectiveAtherosclerosis (AS) is the major cause of cardiovascular disease, and dyslipidemia is a principal determinant of the initiation and progression of AS. Numerous works have analyzed the lipid signature of blood, but scarce information on the lipidome of vascular tissue is available. This study investigated the lipid profile in the aorta of ApoE−/− mice.MethodApoE−/− mice were randomly divided into two groups: (1) the normal diet (ND) group and (2) the high-fat diet (HFD) group. After feeding for 8 weeks, the plasma low-density lipoprotein (LDL), total cholesterol (TC), and triglyceride (TGs) levels were measured. UHPLC-Q Exactive plus MS was used to assess the lipid profile using both positive and negative ionization modes.ResultsLDL and TC levels were significantly increased in HFD mice, and lipid deposition, plaque area and collagen fiber levels were increased in HFD group. In addition, a total of 131 differential lipids were characterized, including 57 lipids with levels that were increased in the HFD group and 74 with levels that were decreased. Further analysis revealed that the levels of several differentially expressed phosphocholines (PCs) and lysophosphocholines (LPCs) were significantly increased. These PCs included PC (38:3), PC (36:4), PC (36:3), PC (36:2), PC (36:1), PC (34:1e), PC (34:1), PC (32:1), PC (18:0/18:1), and PC (38:5), and the LPCs included LPC (18:1), LPC (18:0) and LPC (16:0).ConclusionOur findings indicate the presence of a comprehensive lipid profile in the vascular tissue of atherosclerotic mice, particularly involving PC and LPC, which exhibited significantly increased levels in AS.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 3

Fig. 1

Fig. 3

Fig. 1

40798_2022_490_Article_IEq46.gif

Fig. 5

Fig. 57