The effect of type-2 diabetes conditions on neutrophil rolling adhesion

BMC Research Notes

Research Note

Isaac T. S. Li¹  Keith Taverner¹  Adam B. Yasunaga¹  Christine Furrer¹  Yousif Murad²  Jonathan Little³ 

[1] Department of Chemistry, University of British Columbia Okanagan, 3247 University Way, V1V 1V7, Kelowna, BC, Canada;Department of Chemistry, University of British Columbia Okanagan, 3247 University Way, V1V 1V7, Kelowna, BC, Canada;Faculty of Medicine, University of British Columbia, Kelowna, BC, Canada;School of Health and Exercise Sciences, University of British Columbia, Kelowna, BC, Canada;

关键词: HL-60;    Neutrophils;    Type-2 diabetes mellitus;    Cell rolling adhesion;    P-selectin;    PSGL-1;   

DOI  :  10.1186/s13104-022-06248-0

 received in 2022-06-08, accepted in 2022-11-14,  发布年份 2022

来源: Springer

【 摘 要 】

ObjectiveType 2 diabetes mellitus (T2D) is the result of a dysregulation of insulin production and signalling, leading to an increase in both glucose concentration and pro-inflammatory cytokines such as interleukin (IL)-6 and tumour necrosis factor (TNF)-α. Previous work showed that T2D patients exhibited immune dysfunction associated with increased adhesion molecule expression on endothelial cell surfaces, accompanied by decreased neutrophil rolling velocity on the endothelial cell surface. Changes in cell rolling adhesion have direct vascular and immune complications such as atherosclerosis and reduced healing time in T2D patients. While previous studies focused primarily on how endothelial cells affect neutrophil rolling under T2D conditions, little is known about changes to neutrophils that affect their rolling. In this study, we aim to show how the rolling behaviour of neutrophils is affected by T2D conditions on a controlled substrate.ResultsWe found that neutrophils cultured in T2D-serum mimicking media increased cell rolling velocity compared to neutrophils under normal conditions. Specifically, glucose alone is responsible for higher rolling velocity. While cytokines further increase the rolling velocity, they also reduce the cell size. Both glucose and cytokines likely reduce the function of P-selectin Glycoprotein Ligand-1 (PSGL-1) on neutrophils.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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