Auricular cartilage regeneration using different types of mesenchymal stem cells in rabbits

Biological Research

Research Article

Mohamed Ahmed Maher¹  Taghreed Ahmed Hassan¹  Hamdy Rizk¹  Ayman Tolba Reyad¹  Amr Fekry El Karmoty¹  Zainab Sabry Othman Ahmed²  Marwa A Ibrahim³ 

[1] Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza Square, 12211, Giza, Egypt;Cytology and Histology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;King Salman International University, South Sinai, Ras Sudr, Egypt;Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza Square, 12211, Giza, Egypt;

关键词: Mesenchymal stem cells;    Auricular cartilage defects;    Bone marrow;    Ear;    Rabbit;   

DOI  :  10.1186/s40659-022-00408-z

 received in 2022-09-15, accepted in 2022-12-13,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundCartilaginous disorders comprise a wide range of diseases that affect normal joint movement, ear and nose shape; and they have great social and economic impact. Mesenchymal stem cells (MSCs) provide a promising regeneration alternative for treatment of degenerative cartilaginous disorders. This study aimed to compare therapeutic potential of different types of laser activated MSCs to promote auricular cartilage regeneration. Twelve adult rabbit allocated equally in four groups, all animals received a surgical mid auricular cartilage defect in one ear; Group I (Positive control) injected sub-perichondrially with phosphate-buffered saline (PBS), Group II (ADMSC-transplanted group) injected adipose-derived MSCs (ADMSCs), Group III (BMMSCs-transplanted group) received bone marrow-derived MSCs (BMMSCs), and Group IV (EMSC-transplanted group) received ear MSCs (EMSCs) in the defected ear. The auricular defect was analyzed morphologically, histopathologically and immunohistochemically after 4 weeks. In addition, a quantitative real-time polymerase chain reaction was used to examine expression of the collagen type II (Col II) and aggrecan as cartilage growth factors.ResultsThe auricles of all treatments appeared completely healed with smooth surfaces and similar tissue color. Histopathologically, defective areas of control positive group, ADMSCs and EMSCs treated groups experienced a small area of immature cartilage. While BMMSCs treated group exhibited typical features of new cartilage formation with mature chondrocytes inside their lacunae and dense extracellular matrix (ECM). In addition, BMMSC treated group showed a positive reaction to Masson’s trichrome and orcein stains. In contrary, control positive, ADMSC and EMSC groups revealed faint staining with Masson’s trichrome and Orcein. Immunohistochemically, there was an intense positive S100 expression in BMMSCs (with a significant increase of area percentage + 21.89 (P < 0.05), a moderate reaction in EMSCs (with an area percentage + 17.97, and a mild reaction in the control group and ADMSCs (area percentages + 8.02 and + 11.37, respectively). The expression of relative col II and aggrecan was substantially highest in BMMSCs (± 0.91 and ± 0.89, respectively). While, Control positive, ADMSCs and EMSCs groups recorded (± 0.41: ± 0.21, ± 0.6: ± 0.44, ± 0.61: ± 0.63) respectively.ConclusionBMMSCs showed the highest chondrogenic potential compared to ADMSCs and EMSCs and should be considered the first choice in treatment of cartilaginous degenerative disorders.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

12902_2022_1244_Article_IEq19.gif

12982_2022_119_Article_IEq163.gif

12888_2022_4365_Article_IEq45.gif

12982_2022_119_Article_IEq238.gif

12982_2022_119_Article_IEq223.gif

12982_2022_119_Article_IEq78.gif

12982_2022_119_Article_IEq203.gif

12888_2022_4392_Article_IEq3.gif

12888_2022_4392_Article_IEq1.gif