CD19+CD24highCD27+ B cell and interleukin 35 as potential biomarkers of disease activity in systemic lupus erythematosus patients

Advances in Rheumatology

Research

Ying Xu¹  Zhenrui Shi²  Qing Guo²  Zengqi Tang²  Guozhen Tan²  Xiuting Liu²  Hui Xiong³  Xuechen Ai⁴  Zhixuan Guo⁵ 

[1] Department of Clinical Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 510120, Guangzhou, Guangdong, China;Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 510120, Guangzhou, Guangdong, China;Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 510120, Guangzhou, Guangdong, China;Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetic and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 510120, Guangzhou, Guangdong, China;Department of Dermatology, The Eighth Affiliated Hospital, Sun Yat-sen University, 518033, Shenzhen, Guangdong, China;Department of Dermatology, The Seventh Affiliated Hospital, Sun Yat-sen University, 518040, Shenzhen, Guangdong, China;

关键词: Systemic lupus erythematosus;    Regulatory B cells;    Interleukin 35;    Interleukin 10;   

DOI  :  10.1186/s42358-022-00279-8

 received in 2021-10-12, accepted in 2022-11-19,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundSystemic lupus erythematosus (SLE) is a systemic autoimmune disease that associates with aberrant activation of B lymphocytes and excessive autoantibodies. Interleukin 10 (IL-10)/interleukin 35 (IL-35) and IL-10/IL-35-producing regulatory B cells have been demonstrated to possess immunosuppressive functions during systemic lupus erythematosus. Here, we detected the proportion of CD19+CD24highCD27+ B cells as well as IL-10 and IL-35 levels in peripheral blood of SLE patients and healthy individuals, and investigated their relations with clinical features of SLE.Methods41 SLE patients and 25 healthy controls were recruited. The patients were divided into groups based on SLEDAI score, anti-dsDNA antibody, rash, nephritis and hematological disorder. Flow cytometry was used to detect the proportion of CD24hiCD27+ B cells. ELISA was used to detect serum levels of IL-10 and IL-35.ResultsOur results showed that the CD19+CD24highCD27+ B population was decreased in active SLE patients, and anti-correlated with the disease activity. Of note, we found significant increase of IL-10 and decrease of IL-35 in SLE patients with disease activity score > 4, lupus nephritis or hematological disorders compared to those without related clinical features.ConclusionsReduced CD19+CD24highCD27+ B cells expression may be involved in the pathogenesis of SLE. Moreover, we supposed that IL-35 instead of IL-10 played a crucial role in immune regulation during SLE disease.

【 授权许可】

CC BY   
© The Author(s) 2022

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