Gut microbiota in the early stage of Crohn’s disease has unique characteristics

Gut Pathogens

Research

Wenyu Zhang¹  Shu Li²  Yan Jia²  Xiaojuan Lu²  Hui Xie²  Mingjie Zhang²  Yuqi He²  Xin Wang²  Peng Jin³  Lang Yang³  Xianzong Ma⁴  Dezhi Wang⁴  Jianqiu Sheng⁵  Junfeng Xu⁶ 

[1]Capital Medical University, 100069, Beijing, China

[2]Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, No.5 Nanmencang, 100700, Beijing, China

[3]Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, No.5 Nanmencang, 100700, Beijing, China

[4]Senior Department of Gastroenterology, The First Medical Center of Chinese PLA General Hospital, No.28 Fuxing Road, 100853, Beijing, China

[5]Medical School of Chinese PLA, 100853, Beijing, China

[6]Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, No.5 Nanmencang, 100700, Beijing, China

[7]Medical School of Chinese PLA, 100853, Beijing, China

[8]Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, No.5 Nanmencang, 100700, Beijing, China

[9]Senior Department of Gastroenterology, The First Medical Center of Chinese PLA General Hospital, No.28 Fuxing Road, 100853, Beijing, China

[10]Senior Department of Gastroenterology, The First Medical Center of Chinese PLA General Hospital, No.28 Fuxing Road, 100853, Beijing, China

关键词: Crohn’s disease;    Early diagnosis;    Gut microbiota;    16S rDNA;    Dysbacteriosis;   

DOI  :  10.1186/s13099-022-00521-0

 received in 2022-06-20, accepted in 2022-12-06,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundEmerging evidence suggests that gut microbiota plays a predominant role in Crohn’s disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore specific gut bacteria related to the progression of CD.MethodsThis study was nested within a longitudinal prospective Chinese CD cohort, and it included 18 early CD patients, 22 advanced CD patients and 30 healthy controls. The microbiota communities were investigated using high-throughput Illumina HiSeq sequencing targeting the V3–V4 region of 16S ribosomal DNA (rDNA) gene. The relationship between the gut microbiota and clinical characteristics of CD was analyzed.ResultsDifferential microbiota compositions were observed in CD samples (including early and advanced CD samples) and healthy controls samples. Notably, Lachnospiracea_incertae_sedis and Parabacteroides were enriched in the early CD patients, Escherichia/Shigella, Enterococcus and Proteus were enriched in the advanced CD patients, and Roseburia, Gemmiger, Coprococcus, Ruminococcus 2, Butyricicoccus, Dorea, Fusicatenibacter, Anaerostipes, Clostridium IV were enriched in the healthy controls [LDA score (log10) > 2]. Furthermore, Kruskal–Wallis Rank sum test results showed that Blautia, Clostridium IV, Coprococcus, Dorea, Fusicatenibacter continued to significantly decrease in early and advanced CD patients, and Escherichia/Shigella and Proteus continued to significantly increase compared with healthy controls (P < 0.05). The PICRUSt analysis identified 16 remarkably different metabolic pathways [LDA score (log10) > 2]. Some genera were significantly correlated with various clinical parameters, such as fecal calprotectin, erythrocyte sedimentation rate, C-reactive protein, gland reduce, goblet cells decreased, clinical symptoms (P < 0.05).ConclusionsDysbacteriosis occurs in the early stage of CD and is associated with the progression of CD. This data provides a foundation that furthers the understanding of the role of gut microbiota in CD’s pathogenesis.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

12982_2022_119_Article_IEq207.gif

12982_2022_119_Article_IEq74.gif

12982_2022_119_Article_IEq68.gif

12982_2022_119_Article_IEq65.gif

12982_2022_119_Article_IEq53.gif

12982_2022_119_Article_IEq201.gif