Noninvasive prenatal diagnosis targeting fetal nucleated red blood cells

Journal of Nanobiotechnology

Review

Feng Guo¹  Lu Mao¹  Yanyu Chen²  Qinqin Huang³  Zhuhao Wu³  Zi Chen⁴  Joseph Sutlive⁴  Jiabao Nie⁵  Ke Wu⁶  Xing-Zhong Zhao⁶ 

[1] Academy of Medical Sciences, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450052, Zhengzhou, China;Academy of Medical Sciences, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou University, 450052, Zhengzhou, China;School of Physics and Technology, Wuhan University, 430072, Wuhan, China;Department of Intelligent Systems Engineering, Indiana University, 47405, Bloomington, IN, USA;Division of Thoracic and Cardiac Surgery, Brigham and Women’s Hospital and Harvard Medical School, 02115, Boston, MA, USA;Division of Thoracic and Cardiac Surgery, Brigham and Women’s Hospital and Harvard Medical School, 02115, Boston, MA, USA;Department of Biological Sciences, Northeastern University, 02115, Boston, MA, USA;School of Physics and Technology, Wuhan University, 430072, Wuhan, China;

关键词: Microfluidics;    Microtechnology;    Biosensing;    Noninvasive prenatal diagnosis;    Fetal-nucleated red blood cells;   

DOI  :  10.1186/s12951-022-01749-3

 received in 2022-11-11, accepted in 2022-12-15,  发布年份 2022

来源: Springer

【 摘 要 】

Noninvasive prenatal diagnosis (NIPD) aims to detect fetal-related genetic disorders before birth by detecting markers in the peripheral blood of pregnant women, holding the potential in reducing the risk of fetal birth defects. Fetal-nucleated red blood cells (fNRBCs) can be used as biomarkers for NIPD, given their remarkable nature of carrying the entire genetic information of the fetus. Here, we review recent advances in NIPD technologies based on the isolation and analysis of fNRBCs. Conventional cell separation methods rely primarily on physical properties and surface antigens of fNRBCs, such as density gradient centrifugation, fluorescence-activated cell sorting, and magnetic-activated cell sorting. Due to the limitations of sensitivity and purity in Conventional methods, separation techniques based on micro-/nanomaterials have been developed as novel methods for isolating and enriching fNRBCs. We also discuss emerging methods based on microfluidic chips and nanostructured substrates for static and dynamic isolation of fNRBCs. Additionally, we introduce the identification techniques of fNRBCs and address the potential clinical diagnostic values of fNRBCs. Finally, we highlight the challenges and the future directions of fNRBCs as treatment guidelines in NIPD.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2022

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