Molecular machineries of ciliogenesis, cell survival, and vasculogenesis are differentially expressed during regeneration in explants of the demosponge Halichondria panicea

BMC Genomics

Research

Ana Riesgo¹  Sally P. Leys²  Peter Funch³  Nadia Santodomingo⁴  Michelle M. Leger⁵  Vasiliki Koutsouveli⁶  Lars Kumala⁷ 

[1] Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales (CSIC), Calle José Gutiérrez Abascal 2, 28006, Madrid, Spain;Department of Life Sciences, Natural History Museum, Cromwell Road, SW5 7BD, London, UK;Department of Biological Sciences, University of Alberta, 11455 Saskatchewan Drive, T6G 2R3, Edmonton, Alberta, Canada;Department of Biology, Aarhus University, Ny Munkegade, 114-116, Aarhus C, Denmark;Department of Life Sciences, Natural History Museum, Cromwell Road, SW5 7BD, London, UK;Department of Earth Sciences, Oxford University, South Parks Road, OX1 3AN, Oxford, UK;Institute of Evolutionary Biology (CSIC-UPF), Paseo Marítimo de la Barceloneta 37-49, 08003, Barcelona, Spain;Marine Symbioses Research Unit, GEOMAR Helmholtz Centre for Ocean Research Kiel, Düsternbrooker Weg 20, D-24105, Kiel, Germany;Nordcee, Department of Biology, University of Southern Denmark, Campusvej 55, 5230, Odense M, Denmark;Marine Biological Research Center, University of Southern Denmark, Hindsholmvej 11, 5300, Kerteminde, Denmark;

关键词: Porifera;    Transcriptomics;    Regeneration;    Angiogenesis;   

DOI  :  10.1186/s12864-022-09035-0

 received in 2022-03-22, accepted in 2022-11-21,  发布年份 2022

来源: Springer

【 摘 要 】

Sponges are interesting animal models for regeneration studies, since even from dissociated cells, they are able to regenerate completely. In particular, explants are model systems that can be applied to many sponge species, since small fragments of sponges can regenerate all elements of the adult, including the oscula and the ability to pump water. The morphological aspects of regeneration in sponges are relatively well known, but the molecular machinery is only now starting to be elucidated for some sponge species. Here, we have used an explant system of the demosponge Halichondria panicea to understand the molecular machinery deployed during regeneration of the aquiferous system. We sequenced the transcriptomes of four replicates of the 5–day explant without an osculum (NOE), four replicates of the 17–18–day explant with a single osculum and pumping activity (PE) and also four replicates of field–collected individuals with regular pumping activity (PA), and performed differential gene expression analysis. We also described the morphology of NOE and PE samples using light and electron microscopy. Our results showed a highly disorganised mesohyl and disarranged aquiferous system in NOE that is coupled with upregulated pathways of ciliogenesis, organisation of the ECM, and cell proliferation and survival. Once the osculum is formed, genes involved in “response to stimulus in other organisms” were upregulated. Interestingly, the main molecular machinery of vasculogenesis described in vertebrates was activated during the regeneration of the aquiferous system. Notably, vasculogenesis markers were upregulated when the tissue was disorganised and about to start forming canals (NOE) and angiogenic stimulators and ECM remodelling machineries were differentially expressed once the aquiferous system was in place (PE and PA). Our results are fundamental to better understanding the molecular mechanisms involved in the formation of the aquiferous system in sponges, and its similarities with the early onset of blood-vessel formation in animal evolution.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

12888_2022_4365_Article_IEq38.gif

12888_2022_4365_Article_IEq34.gif

12888_2022_4365_Article_IEq26.gif

12888_2022_4365_Article_IEq23.gif

Fig. 6

12982_2022_119_Article_IEq214.gif

Fig. 3

Fig. 1

12982_2022_119_Article_IEq72.gif

12982_2022_119_Article_IEq205.gif

12888_2022_4137_Article_IEq1.gif

Fig. 5

Fig. 1