Correlation between Genetic Polymorphism of CYP2A13 Genotype and Lung Cancer in Female Passive Smokers

Molecular and Cellular Biomedical Sciences: MCBS

article

Nurul Ramadhani¹  Noni Novisari Soeroso²  Setia Putra Tarigan³  Putri Chairani Eyanoer⁴  Hidayat Hidayat⁵ 

[1] Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara/Adam Malik General Hospital;Division of Thoracic Oncology, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara/Universitas Sumatera Utara Hospital;Division of Thoracic Oncology, Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara/Adam Malik General Hospital;Department of Public Health, Faculty of Medicine, Universitas Sumatera Utara;Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara

关键词: polymorphism;    CYP2A13;    PCR-RFLP;    female passive smoker;    lung cancer;   

DOI  :  10.21705/mcbs.v6i2.246

来源: Cell and BioPharmaceutical Institute

【 摘 要 】

Background: Nicotine is metabolized to cotinine by cytochrome P450 enzyme, and this enzyme is involved in the activation of toxic and carcinogenic substances. The aim of this research was to assess the relationship between genetic polymorphism of CYP2A13 and lung cancer incidence in female passive smokers.Materials and methods: This research was a case-control study that involved 104 research subjects. Subjects were recruited through purposive sampling technique from 2 hospitals in Medan, North Sumatra, Indonesia. The case population consisted of female passive smokers with lung cancer and the control population consisted of female passive smokers without lung cancer. All research subjects underwent blood sampling for genomics DNA extraction and CYP2A13 genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Data was analyzed by conditional logistic regression by Epi Info 7.0 software.Results: Among 104 subjects, 26 (25%) individuals were heterozygous, 76 (73%) individuals were wild type, and 2 (2%) were mutant for the 257Cys allele. There was a significant correlation between CYP2A13 genotype and lung cancer incidence (p-value<0.05). Female passive smokers with CT genotype had 2.7 greater risk of developing lung cancer than those with CC genotype (wild type). The C allele had more frequency and 1.6 times higher risk of lung cancer compared to T allele with a wide confidence range (0.73–3.52).Conclusion: There was a significant correlation between CYP2A13 polymorphism and lung cancer incidence in female passive smokers.

【 授权许可】

Unknown