Bioprospecting of desert actinobacteria with special emphases on griseoviridin, mitomycin C and a new bacterial metabolite producing Streptomyces sp. PU-KB10–4

BMC Microbiology

article

Saleem, Maira¹  Hassan, Ashba¹  Li, Feina⁴  Lu, Qinpei⁴  Ponomareva, Larissa V.²  Parkin, Sean⁵  Sun, Chenghang⁴  Thorson, Jon S.²  Shaaban, Khaled A.²  Sajid, Imran¹ 

[1] Institute of Microbiology and Molecular Genetics ,(MMG), University of the Punjab;Center for Pharmaceutical Research and Innovation ,(CPRI), College of Pharmacy, University of Kentucky;Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky;Institute of Medicinal Biotechnology ,(IMB), Chinese Academy of Medical Sciences & Peking Union Medical College;Department of Chemistry, University of Kentucky

关键词: Kubuqi desert;    Actinobacteria;    Natural products;    Antibacterial;    Anticancer;    Streptomyces;    Mitomycin;    4-hydroxycinnamide;   

DOI  :  10.1186/s12866-023-02770-8

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Bioprospecting of actinobacteria isolated from Kubuqi desert, China for antibacterial, antifungal and cytotoxic metabolites production and their structure elucidation. A total of 100 actinobacteria strains were selectively isolated from Kubuqi desert, Inner Mongolia, China. The taxonomic characterization revealed Streptomyces as the predominant genus comprising 37 different species, along with the rare actinobacterial genus Lentzea. The methanolic extracts of 60.8% of strains exhibited potent antimicrobial activities against Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Escherichia coli, Salmonella enterica, Saccharomyces cerevisiae and high to mild in vitro cytotoxicity against PC3 (prostate cancer) and A549 (lung carcinoma) cell lines. The metabolomics analysis by TLC, HPLC-UV/vis, HPLC-MS and NMR showed the presence of compounds with molecular weights ranging from 100 to 1000 Da. The scale-up fermentation of the prioritized anti-Gram-negative strain PU-KB10–4 (Streptomyces griseoviridis), yielded three pure compounds including; griseoviridin (1; 42.0 mgL− 1) with 20 fold increased production as compared to previous reports and its crystal structure as monohydrate form is herein reported for the first time, mitomycin C (2; 0.3 mgL− 1) and a new bacterial metabolite 4-hydroxycinnamide (3; 0.59 mgL− 1). This is the first report of the bioprospecting and exploration of actinobacteria from Kubuqi desert and the metabolite 4-hydroxycinnamide (3) is first time isolated from a bacterial source. This study demonstrated that actinobacteria from Kubuqi desert are a potential source of novel bioactive natural products. Underexplored harsh environments like the Kubuqi desert may harbor a wider diversity of actinobacteria, particularly Streptomyces, which produce unique metabolites and are an intriguing source to develop medicinally valuable natural products.

【 授权许可】

CC BY|CC0