Differential CXC and CX3C Chemokine ExpressionProfiles in Aqueous Humor of Patients With SpecificEndogenous Uveitic Entities

Investigative Ophthalmology & Visual Science

article

Ahmed M. Abu El-Asrar¹  Nele Berghmans³  Saleh A. Al-Obeidan¹  Priscilla W. Gikandi¹  Ghislain Opdenakker³  Jo Van Damme³  Sofie Struyf³ 

[1] Department of Ophthalmology, College of Medicine, King Saud University;Dr. Nasser Al-Rashid Research Chair in Ophthalmology, King Saud University;Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven

关键词: uveitis;    Beh¸cet’s disease;    sarcoidosis;    HLA-B27;    Vogt-Koyanagi-Harada disease;   

DOI  :  10.1167/iovs.17-23225

学科分类：社会科学、人文和艺术（综合）

来源: Association for Research in Vision and Ophthalmology

【 摘 要 】

PURPOSE. To determine the levels of the neutrophil chemoattractants CXCL1, CXCL2, CXCL5,CXCL6, and CXCL8, the T helper 1 chemoattractants CXCL9, CXCL10 and CXCL11, the lymphoidchemokines CXCL12 and CXCL13 and the soluble form of the transmembrane chemokinesCXCL16 and CX3CL1, in aqueous humor samples from patients with specific uveitic entities.METHODS. Aqueous humor samples from patients with active uveitis associated with Beh¸cet’sdisease (n ¼ 13), sarcoidosis (n ¼ 8), HLA-B27–related inflammation (n ¼ 12), Vogt-KoyanagiHarada (VKH) disease (n ¼ 12), and healthy controls (n ¼ 9) were assayed with the use of amultiplex assay.RESULTS. All chemoattractant levels were significantly higher in all patients than in thecontrols. The levels of all neutrophil chemoattractants and CXCL10, CXCL16, and CX3CL1were significantly higher in nongranulomatous uveitis (Beh¸cet’s disease and HLA-B27–associated uveitis) than in granulomatous uveitis (sarcoidosis and VKH disease), whereas thelevels of the B cell chemoattractant CXCL13 were significantly higher in granulomatousuveitis than in nongranulomatous uveitis. CXCL13 levels were highest in the patients withVKH disease. CXCL9, CXCL11, and CXCL12 levels did not differ significantly.CONCLUSIONS. Inflammation in nongranulomatous uveitis appears to be driven by neutrophilsand T helper 1 lymphocytes, whereas B lymphocytes may contribute to the inflammatoryprocess in granulomatous uveitis, particularly in VKH disease.

【 授权许可】

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