Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis

eLife

Melissa Meyer zu Horste¹  Karin Loser²  Tobias Lautwein³  Xiaolin Li³  David Schafflick³  Heinz Wiendl³  Michael Heming³  Gerd Meyer zu Hörste³  Dirk Bauer⁴  Maren Kasper⁴  Karoline Walscheid⁵  Arnd Heiligenhaus⁶ 

[1] Augen-Zentrum-Nordwest Augenpraxis Ahaus, Ahaus, Germany;Department of Human Medicine, University of Oldenburg, Oldenburg, Germany;Department of Neurology with Institute of Translational Neurology, University Hospital Muenster, Muenster, Germany;Ophtha-Lab, Department of Ophthalmology, and Uveitis Centre at St. Franziskus Hospital, Münster, Germany;Ophtha-Lab, Department of Ophthalmology, and Uveitis Centre at St. Franziskus Hospital, Münster, Germany;Department of Ophthalmology, University of Duisburg-Essen, Essen, Germany;Ophtha-Lab, Department of Ophthalmology, and Uveitis Centre at St. Franziskus Hospital, Münster, Germany;University of Duisburg-Essen, Essen, Germany;

关键词: HLA-B27;    uveitis;    inflammation;    dendritic cells;    Human;   

DOI  :  10.7554/eLife.67396

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Uveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. Uveitis associated with the HLA haplotype B27 (HLA-B27) is a common subtype of uveitis and a prototypical ocular immune-mediated disease. Local immune mechanisms driving human uveitis are poorly characterized mainly due to the limited available biomaterial and subsequent technical limitations. Here, we provide the first high-resolution characterization of intraocular leukocytes in HLA-B27-positive (n = 4) and -negative (n = 2) anterior uveitis and an infectious endophthalmitis control (n = 1) by combining single-cell RNA-sequencing with flow cytometry and protein analysis. Ocular cell infiltrates consisted primarily of lymphocytes in both subtypes of uveitis and of myeloid cells in infectious endophthalmitis. HLA-B27-positive uveitis exclusively featured a plasmacytoid and classical dendritic cell (cDC) infiltrate. Moreover, cDCs were central in predicted local cell-cell communication. This suggests a unique pattern of ocular leukocyte infiltration in HLA-B27-positive uveitis with relevance to DCs.

【 授权许可】

CC BY