Mechanical Strain Induces Distinct Human ScleralFibroblast Lineages: Differential Roles in CellProliferation, Apoptosis, Migration, and Differentiation

Investigative Ophthalmology & Visual Science

article

Chen Qiu¹  Minjie Chen¹  Jing Yao¹  Xinghuai Sun¹  Jianjiang Xu¹  Rong Zhang¹  Xin Wang¹  Gang Li¹  Shaohong Qian¹ 

[1] Department of Ophthalmology, Eye and Ear, Nose, Throat Hospital, Shanghai Medical College, Fudan University;Key Laboratory of Myopia, Ministry of Health, Fudan University;State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University;Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University

关键词: mechanical strain;    sclera;    fibroblasts;    cell behavior;    glaucoma;   

DOI  :  10.1167/iovs.18-23855

学科分类：社会科学、人文和艺术（综合）

来源: Association for Research in Vision and Ophthalmology

【 摘 要 】

PURPOSE. The purpose of this study was to explore the effect of mechanical strain on humanscleral fibroblasts (HSFs) and compare cell behaviors of HSFs from distinct regions.METHODS. Primary HSFs were cultivated using a digestive protocol. Cells were seeded oncollagen I–coated Bioflex plates, and a FX-5000 tension system was used to perform biaxialmechanical strain in vitro. We applied 10%, 0.5-Hz mechanical strain. Cell behaviors ofperipapillary and periphery HSFs were compared after the strain. Edu imaging, Cell CountingKit-8 assay, and cell cycle flow cytometry were conducted to analyze cell proliferation ability.For cell apoptosis, flow cytometry of Annexin V/propidium iodide, caspase 3 activity, andWestern blot were performed. Immunofluorescence, real-time PCR, and Western blot wereused to investigate cell differentiation. A migration assay was also performed.RESULTS. Under the mechanical strain of 10%, 0.5 Hz for 24 hours, the proliferation ability andcell apoptosis of peripapillary HSFs did not have a significant change. The expression of alphasmooth muscle actin (a-SMA) slightly decreased. However, increased cell proliferation,attenuated cell apoptosis and more expression of a-SMA were shown in the periphery HSFsunder the same condition. The migration rate was also increased for periphery HSFs, whereasit kept almost the same for peripapillary HSFs under 10%, 0.5-Hz strain for 8 hours.CONCLUSIONS. Mechanical strain affected the cell behaviors of HSFs. The different performanceof cells from distinct regions may suggest familial linages of HSFs, probably induced bymechanical strain.

【 授权许可】

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