Cytocompatible and Antibacterial Properties of Chitosan-Siloxane Hybrid Spheres

Polymers

Saki Yasutomi¹  Yuki Shirosaki¹  Akiyoshi Osaka²  Satoshi Hayakawa³  Susana Cruz-Neves⁴  Toshinari Maeda⁴  Toshiki Miyazaki⁴  Manato Nakatsukasa⁵ 

[1] Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka 804-8550, Japan;Faculty of Engineering, Okayama University, 3-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan;Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan;Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0196, Japan;Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan;

关键词: chitosan-siloxane hybrid;    microporous spheres;    cell behavior;    cerium ion;    antibacterial property;   

DOI  :  10.3390/polym11101676

来源: DOAJ

【 摘 要 】

Microporous spheres in a hybrid system consisting of chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols, with or without a cerium chloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 μm. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 μm in diameter, whereas MG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.

【 授权许可】

Unknown