Stem cell sheet fabrication from human umbilical cord mesenchymal stem cell and Col-T scaffold

Stem cell research

article

Truc Le-Buu Pham¹  Tram Mai Nguyen²  Dang Phu-Hai Nguyen³  Huynh Nhu Tran¹  Tam Thi-Thanh Nguyen¹  Nguyen Trong Binh¹  Quan Dang Nguyen¹  Hong-Thuy Bui³ 

[1] Biotechnology Center of Ho Chi Minh City 700000;University of Copenhagen;International University, Vietnam National University

关键词: Cell sheet;    Col-T scaffold;    Mesenchymal stem cells;    Regenerative medicine;    Umbilical cord;   

DOI  :  10.1016/j.scr.2022.102960

学科分类：生理学

来源: Academic Press

【 摘 要 】

Today, stem cell therapy has been shown to be a remarkable progress and an important application in the regeneration of defective tissues and organs. To deliver stem cells to the injured area, several methods have been proposed such as an intravenous infusion, direct damaged tissue injection, or stem cell sheet transplantation. In this study, we aimed to fabricate a stem cell sheet by culturing human umbilical cord mesenchymal stem cells (hUC-MSCs) on a Col-T scaffold to recover the structure and function of damaged tissues. The results showed that cells reach confluent on the scaffold surface 18 h after seeding. These stem cells were able to survive and proliferate on Col-T scaffold. The average tensile strength of the stem cell sheet was 2.65 MPa. The sheet reached the sterile standards when tested for total bacteria, Candida albicans , Pseudomonas aeruginosa , and Staphylococcus aureus according to Circular number 06/2011/TT-BYT of Vietnam Ministry of Health. In addition, the stem cell sheet was non-toxic when evaluated for exposure toxicity and fluid toxicity according to iSO-10993. Importantly, 5 days after culturing on the Col-T scaffold, the seeded hUC-MSCs were still possessed all properties of MSC such as spindle-shaped, adhesive, could differentiate into mesoderm-derived cells, showed to be CD90, CD105, CD73 positive and CD45, CD34, CD11b, CD19, HLA-DR negative. In summary, our study was successful in creating a stem cell sheet from hUC-MSCs and Col-T scaffold for subsequent in vivo transplantation in the future.

【 授权许可】

CC BY|CC BY-NC-ND