Inverse opal scaffolds and photoacoustic microscopy for regenerative medicine

Tissue engineering;Regenerative medicine;Inverse opal scaffolds;Uniform;Poly(D,L-lactic-co-glycolic acid) (PLGA);Photoacoustic microscopy;Biomedical Imaging

Zhang, Yu ; Xia, Younan Biomedical Engineering (Joint GT/Emory Department) Wang, Lihong V. Temenoff, Johnna S. Barker, Thomas H. Botchwey, Edward A. Lyon, Andrew ; Xia, Younan

University:Georgia Institute of Technology

Department:Biomedical Engineering (Joint GT/Emory Department)

关键词: Tissue engineering;    Regenerative medicine;    Inverse opal scaffolds;    Uniform;    Poly(D,L-lactic-co-glycolic acid) (PLGA);    Photoacoustic microscopy;    Biomedical Imaging;   

Others  :  https://smartech.gatech.edu/bitstream/1853/50231/1/ZHANG-DISSERTATION-2013.pdf

美国|英语

来源: SMARTech Repository

【 摘 要 】

This research centers on the fabrication, characterization, and engineering of inverseopal scaffolds, a novel class of three-dimensional (3D) porous scaffolds made ofbiocompatible and biodegradable polymers, for applications in tissue engineering andregenerative medicine. The unique features of an inverse opal scaffold include a highlyordered array of pores, uniform and finely tunable pore sizes, high interconnectivity, andgreat reproducibility.The first part of this work focuses on the fabrication and functionalization of inverseopal scaffolds based on poly(D,L-lactic-co-glycolic acid) (PLGA), a biodegradablematerial approved by the U.S. Food and Drug Administration (FDA). The advantages ofthe PLGA inverse opal scaffolds are also demonstrated by comparing with theircounterparts with spherical but non-uniform pores and poor interconnectivity.The second part of this work shows two examples where the PLGA inverse opalscaffolds were successfully used as a well-defined system to investigate the effect of poresize of a 3D porous scaffold on the behavior of cell and tissue growth. Specifically, Ihave demonstrated that i) the differentiation of progenitor cells in vitro was dependent onthe pore size of PLGA-based scaffolds and the behavior of the cells was determined bythe size of individual pores where the cells resided in, and ii) the neovascularizationprocess in vivo could be directly manipulated by controlling a combination of pore andwindow sizes when they were applied to a mouse model.The last part of this work deals with the novel application of photoacousticmicroscopy (PAM), a volumetric imaging modality recently developed, to tissueengineering and regenerative medicine, in the context of non-invasive imaging andquantification of cells and tissues grown in PLGA inverse opal scaffolds, both in vitroand in vivo. Furthermore, the capability of PAM to monitor and quantitatively analyzethe degradation of the scaffolds themselves was also demonstrated.