Microarray microRNA profiling of urinary exosomes in a 5XFAD mouse model of Alzheimer’s disease

Animal Models and Experimental Medicine

article

Zhiqi Song¹  Yajin Qu¹  Yanfeng Xu¹  Ling Zhang¹  Li Zhou¹  Yunlin Han¹  Wenjie Zhao¹  Pin Yu¹  Yu Zhang¹  Xianglei Li¹  Chuan Qin¹ 

[1] Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College

关键词: 5XFAD mouse model;    Alzheimer's disease;    biomarkers;    microarray;    miRNA;    urinary exosome;   

DOI  :  10.1002/ame2.12175

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Background Alzheimer's disease (AD) is an incurable and irreversible neurodegenerative disease, without a clear pathogenesis. Therefore, identification of candidates before amyloid-β plaque (Aβ) deposition proceeds is of major significance for earlier intervention in AD. Methods To explore the potential noninvasive earlier biomarkers of AD in a 5XFAD mouse model, microRNAs (miRNAs) from urinary exosomes in 1-month-old pre-Aβ accumulation 5XFAD mice models and their littermate controls were profiled by microarray analysis. The differentially expressed miRNAs were further analyzed via droplet digital PCR (ddPCR). Results Microarray analysis demonstrated that 48 differentially expressed miRNAs (18 upregulated and 30 downregulated), of which six miRNAs – miR-196b-5p, miR-339-3p, miR-34a-5p, miR-376b-3p, miR-677-5p, and miR-721 – were predicted to display gene targets and important signaling pathways closely associated with AD pathogenesis and verified by ddPCR. Conclusions Urinary exosomal miRNAs showing differences in expression prior to Aβ-plaque deposition were identified. These exosomal miRNAs represent potential noninvasive biomarkers that may be used to prevent AD in clinical applications.

【 授权许可】

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