Biological synthesis of Fe 3 O 4 @Ag using Scenedesmus obliquus and evaluation of its effect on the expression of MexA-B efflux pump genes in ciprofloxacin-resistant Pseudomonas aeruginosa strains

Micro & nano letters

article

Sara Hadad¹  Tabarek Abdulrazaq Alkinani²  Aida Mahmoudi³  Somayeh Dehghan⁴  Nastaran Maleki Moaf⁵  Akram Sadat Naeemi⁶  Ali Salehzadeh¹ 

[1] Department of Biology, Rasht Branch, Islamic Azad University;Department of Biology, Science and Research Branch, Islamic Azad University;Department of Biology, Damghan Branch, Islamic Azad University;Department of Biology, Central Tehran Branch, Islamic Azad University;Department of Biology, Lahijan Branch, Islamic Azad University;Department of Biology, Faculty of Science, University of Guilan

关键词: Ciprofloxacin;    Efflux pump;    Nanocomposite;    Pseudomonas aeruginosa;   

DOI  :  10.1049/mna2.12138

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

This study was conducted to biosynthesize Fe3O4@Ag nanocomposite with Scenedesmus obliquus extract, and to evaluate its antibacterial potential against Pseudomonas aeruginosa . In addition, the effect of the synthesized nanocomposite on the expression of MexA-B efflux pump genes was investigated. The Fe 3 O 4 @Ag nanocomposite was characterized using X-ray diffraction spectroscopy (EDX), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), Dynamic light scattering (DLS) as well as Zeta potential analyses. Minimum inhibitory concentration (MIC) of the nanocomposite alone and in combination with ciprofloxacin against P. aeruginosa strains was determined. Finally, the expression of MexA-B efflux pump genes among the bacteria treated with the nanocomposite was evaluated using quantitative real-time PCR. Based on the results, the proper synthesis of the nanocomposite with the diameter of 40 to 70 nm and acceptable stability was confirmed. The synthesized nanocomposite had lower MIC value (8–64 μg/mL) than ciprofloxacin (64–512 μg/mL) which shows stronger antibacterial potential of the nanocomposite. In addition, treating bacteria with the nanocomposite and ciprofloxacin caused a significant reduction of MexA-B genes compared to the cells treated with ciprofloxacin or nanocomposite alone. Thus, Fe 3 O 4 @Ag nanocomposite can improve the efficacy of ciprofloxacin against P. aeruginosa by reducing the expression of MexA-B efflux pump genes.

【 授权许可】

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