Galectin-1–RNA interaction map reveals potential regulatory roles in angiogenesis

FEBS Letters

article

Jiajun Wei¹  Daniel K. Li²  Xinyu Hu³  Chao Cheng⁴  Yi Zhang⁴ 

[1] Department of Neurology, Renmin Hospital of Wuhan University;Department of Biology and Biotechnology, School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology;Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology;ABLife BioBigData Institute

关键词: angiogenesis;    galectin-1;    iRIP-seq;    LGALS1;    RNA-binding;    VEGFA;   

DOI  :  10.1002/1873-3468.14047

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hyperactive angiogenesis contributes to the immunosuppressive microenvironment important for immunotherapy. Galectin-1, encoded by LGALS1 , can trigger the vascular signaling programs and mediate the anti-angiogenic treatment response. However, the mechanism through which galectin-1 regulates angiogenesis is poorly understood. It has been suggested that galectin-1 may associate with mRNAs in cells. This study applied the iRIP-seq methodology to study the potential role of galectin-1 as an RNA-binding protein. We found that galectin-1 interacts with a large number of mRNAs, with a preference for binding near stop codons and a preference for UGCA/UGGA and GAGCAG as binding motifs. Galectin-1 binds to the mRNAs of angiogenesis-associated genes including VEGFA , EGR1, and LAMA5 , suggesting that galectin-1 may regulate angiogenesis via its mRNA-binding activity. We further show that sh LGALS1 inhibits capillary tube formation in an in vitro angiogenesis assay and alters the expression levels of several galectin-1-bound angiogenesis-associated mRNAs. These results uncover a previously unrecognized mRNA-binding activity of galectin-1.

【 授权许可】

Unknown