FEBS Letters | |
PARP1–RNA interaction analysis: PARP1 regulates the expression of extracellular matrix-related genes in HK-2 renal proximal tubular epithelial cells | |
article | |
Jing Ke1  Feng Liu1  Yafang Tu1  Zhitao Cai1  Yu Luo1  Xiongfei Wu1  | |
[1] Department of Nephrology, Renmin Hospital of Wuhan University | |
关键词: extracellular matrix; HK-2 cells; iRIP-seq; PARP1; RNA-seq; | |
DOI : 10.1002/1873-3468.14065 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Recent studies suggest that Poly(ADP-ribose) polymerase 1 (PARP1) acts as an RNA-binding protein in a majority of renal diseases with tubular cell injury. However, detailed knowledge of RNA targets and the RNA-binding regions for PARP1 is unknown. Herein, mapping of iRIP-seq reads in HK-2 renal tubular epithelial cells showed a biased distribution at coding sequence (CDS) and intron regions that is specific to these cells. A total of 1708 differentially expressed genes were identified after PARP1 knockdown using RNA-seq. Furthermore, transcriptome analysis also showed that selective variable splicing was globally regulated by PARP1 in HK-2 cells. By comparison of PARP1 RNA-seq and iRIP-seq data, we found 68 overlapping genes that are enriched in ‘extracellular matrix’ pathway. Follow-up identification of their interactions may contribute vital insights into the regulatory role of PARP1 as an RNA-binding protein in HK-2 cells.
【 授权许可】
Unknown
【 预 览 】
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