PARP1–RNA interaction analysis: PARP1 regulates the expression of extracellular matrix-related genes in HK-2 renal proximal tubular epithelial cells

FEBS Letters

article

Jing Ke¹  Feng Liu¹  Yafang Tu¹  Zhitao Cai¹  Yu Luo¹  Xiongfei Wu¹ 

[1] Department of Nephrology, Renmin Hospital of Wuhan University

关键词: extracellular matrix;    HK-2 cells;    iRIP-seq;    PARP1;    RNA-seq;   

DOI  :  10.1002/1873-3468.14065

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recent studies suggest that Poly(ADP-ribose) polymerase 1 (PARP1) acts as an RNA-binding protein in a majority of renal diseases with tubular cell injury. However, detailed knowledge of RNA targets and the RNA-binding regions for PARP1 is unknown. Herein, mapping of iRIP-seq reads in HK-2 renal tubular epithelial cells showed a biased distribution at coding sequence (CDS) and intron regions that is specific to these cells. A total of 1708 differentially expressed genes were identified after PARP1 knockdown using RNA-seq. Furthermore, transcriptome analysis also showed that selective variable splicing was globally regulated by PARP1 in HK-2 cells. By comparison of PARP1 RNA-seq and iRIP-seq data, we found 68 overlapping genes that are enriched in ‘extracellular matrix’ pathway. Follow-up identification of their interactions may contribute vital insights into the regulatory role of PARP1 as an RNA-binding protein in HK-2 cells.

【 授权许可】

Unknown