Assessment of Extramammary Paget Disease by Two-Photon Microscopy

Frontiers in Medicine

article

Radu Hristu¹  Lucian G. Eftimie¹  Stefan G. Stanciu¹  Remus R. Glogojeanu³  Pavel Gheorghita¹  George A. Stanciu¹ 

[1] Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest;Pathology Department, Central University Emergency Military Hospital;Department of Special Motricity and Medical Recovery, The National University of Physical Education and Sports;Faculty of Energetics, University Politehnica of Bucharest

关键词: second harmonic generation;    two-photon excited fluorescence;    extramammary Paget disease;    immunohistochemistry;    hematoxylin and eosin;   

DOI  :  10.3389/fmed.2022.839786

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Two-photon microscopy techniques are non-linear optical imaging methods which are gaining momentum in the investigation of fixed tissue sections, fresh tissue or even for in vivo experiments. Two-photon excited fluorescence and second harmonic generation are two non-linear optical contrast mechanisms which can be simultaneously used for offering complementary information on the tissue architecture. While the former can originate from endogenous autofluorescence sources (e.g., NADH, FAD, elastin, keratin, lipofuscins, or melanin), or exogenous eosin, the latter is generated in fibrillar structures within living organisms (e.g., collagen and myosin). Here we test the ability of both these contrast mechanisms to highlight features of the extramammary Paget disease on fixed tissue sections prepared for standard histological examination using immunohistochemical markers and hematoxylin and eosin staining. We also demonstrate the label-free abilities of both imaging techniques to highlight histological features on unstained fixed tissue sections. The study demonstrated that two-photon microscopy can detect specific cellular features of the extramammary Paget disease in good correlation with histopathological results.

【 授权许可】

CC BY