MULTIPHOTON MICROSCOPIC IMAGING OF MOUSE INTESTINAL MUCOSA BASED ON TWO-PHOTON EXCITED FLUORESCENCE AND SECOND HARMONIC GENERATION

Journal of Innovative Optical Health Sciences

NING HE¹  HONGSHENG LI²  REN'AN XU²  JIAN XU²  JIANXIN CHEN²  SHUANGMU ZHUO²  SHANGHAI JIANG²  XIAOQIN ZHU² 

[1] Department of Colorectal Surgery, The Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, P. R. China;Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, P. R. China;

关键词: Multiphoton microscopy;    second harmonic generation;    two-photon excited fluorescence;    mucosa;    intestine;    colonic;    duodenal;   

DOI  :  10.1142/S1793545813500041

来源: DOAJ

【 摘 要 】

Multiphoton microscopy (MPM), based on two-photon excited fluorescence and second harmonic generation, enables direct noninvasive visualization of tissue architecture and cell morphology in live tissues without the administration of exogenous contrast agents. In this paper, we used MPM to image the microstructures of the mucosa in fresh, unfixed, and unstained intestinal tissue of mouse. The morphology and distribution of the main components in mucosa layer such as columnar cells, goblet cells, intestinal glands, and a little collagen fibers were clearly observed in MPM images, and then compared with standard H&E images from paired specimens. Our results indicate that MPM combined with endoscopy and miniaturization probes has the potential application in the clinical diagnosis and in vivo monitoring of early intestinal cancer.

【 授权许可】

Unknown