Chronic arthritis directly induces quantitative and qualitative bone disturbances leading to compromised biomechanical properties

Clinical and Experimental Rheumatology

Mário Viana Queiroz¹  Margarida Souto-Carneiro¹  Helena Canhão¹  Ana Margarida Nery¹  Joana Duarte¹  Joana Caetano-Lopes¹  Pamela Weinmann¹  Mário Vale¹  Yrjo Konttinen¹  Paulo Rego¹  João Eurico Fonseca¹  Rita Moura¹  Pedro M Amaral¹  Shimon Sakagushi¹  Patrícia A Pereira¹  Luis Graça¹  Jacinto Monteiro¹  Maria Fátima Vaz¹  Ricardo Henriques¹  Saba Abdulghani¹ 

关键词: Rheumatoid Arthritis;    Osteoporosis;    bone;    SKG mice;    Mechanical tests;    Multiphoton microscopy;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES: Rheumatoid arthritis (RA) is associated with an increased risk of fragility fractures. In RA patients, the direct effect of inflammation on bone is difficult to study because their skeleton is also affected by medication with corticosteroids and other drugs as well as aging and menopause, which contribute to bone fragility. This study used an animal model of chronic arthritis to evaluate the direct impact of chronic inflammation on biomechanical properties and structure of bone. METHODS: In the SKG mouse chronic arthritis model three point bending tests were performed on femoral bones and compression tests on vertebral bodies. Collagen structure was analysed using second-harmonic generation (SHG) imaging with a two-photon microscope, ultramorphology by scanning electron microscopy (SEM) coupled with energy dispersive x-ray spectroscopy (EDS) and bone density using water pycnometer. RESULTS: Arthritic bones had poor biomechanical quality compared to control bones. SHG, SEM and pycnometry disclosed variable signs of impaired collagen organization, poor trabecular architecture and low bone density. CONCLUSIONS: Present data demonstrate for the first time that chronic inflammation per se, without confounding influence of drugs and aging, leads to impairment of bone biomechanics in terms of stiffness, ductility and ultimate strength (fracture).

【 授权许可】

Unknown