Dietary nano-calcium phosphate and hydrolyzed collagen for the inhibition of osteoporosis in calcium-deficient bones

2nd International Conference on Current Progress in Functional Materials 2017

Sapundani, R.^1 ; Juwono, A.L.^1 ; Soejoko, D.S.^1 ; Astuti, D.A.^2

Department of Physics, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok

16424, Indonesia^1

Department of Nutrition and Feed Technology, Faculty of Animal Science, Institut Pertanian Bogor (IPB), Kampus Dramaga, Bogor

16680, Indonesia^2

关键词: Atomic absorption;    Calcium deficients;    Hydrolyzed collagens;    Infrared spectrum;    Nano-calcium phosphates;    Osteoporosis;    Spectrophotometric analysis;    Tri-calcium phosphates;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/496/1/012017/pdf
DOI  :  10.1088/1757-899X/496/1/012017

来源: IOP

【 摘 要 】

Several studies exhibit that high calcium intake prevents osteoporosis-related bone loss. In this study, the dietary effects of nano-tricalcium phosphate (n-CaP) and hydrolyzed collagen (HC) against bone loss at the peak of the bone growth stage are examined, and the main objectives are to prevent osteoporosis and to induce bone formation. The Sprague Dawley mice (Rattus norvegicus) is observed to be calcium deficient (the average calcium content of serum is ≤ 8%). The mice were randomized into four groups (P1: normal purified diet, P2: HC purified diet, P3: n-CaP purified diet, and P4: n-CaP and HC purified diet). After four weeks, all the groups were euthanized, and their bone formation was analyzed. UV-Vis analysis revealed that the calcium content values were 8.20 (P1), 8.92 (P2), 9.2 (P3), and 10.35 (P4) mg/dL, respectively. The corresponding values obtained by the atomic absorption spectrophotometric analysis are 72.29 (P1), 74.25 (P2), 74.80 (P3), and 76.04 (P4) w/w% respectively. To assess the spinal calcification, the infrared spectra were recorded. The splitting factors were 2.5 (P1), 2.6 (P2), 2.5 (P3), and 2.6 (P4), indicating better absorption of n-CaP for P4. Hence, n-CaP and HC-purified diets are suitable to ensure bone growth.