Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia

Frontiers in Public Health

article

Bedru Hussien¹  Aboma Zewude²  Biniam Wondale⁴  Awraris Hailu⁵  Gobena Ameni³ 

[1] Department of Public Health, Goba Referral Hospital, Madda Walabu University;Malaria and Neglected Tropical Diseases Research Team, Ethiopian Public Health Institute, Ministry of Health;Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University;Department of Biology, Arba Minch University, Arba Minch;Department of Public Health, College of Health Sciences, Debre Birhan University;Aklilu Lemma Institute of Pathobiology, Addis Ababa University

关键词: Mycobacterium tuberculosis;    spoligotyping;    clustering;    orphan;    singleton;    Ethiopia;    shared type;   

DOI  :  10.3389/fpubh.2022.808626

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Background Tuberculosis (TB) is a leading cause of morbidity and mortality in Ethiopia. Investigation of the Mycobacterium tuberculosis complex (MTBC) species circulating in the Ethiopian population would contribute to the efforts made to control TB in the country. Therefore, this study was conducted to investigate the MTBC species and spoligo patterns in the Oromia region (central) of Ethiopia. Methods A cross-sectional study design was used to recruit 450 smear positive pulmonary TB (PTB) cases from the Oromia region between September 2017 and August 2018. Mycobacteria were isolated from sputum samples on the Lowenstein Jensen (LJ) medium. Molecular identification of the isolates was performed by spoligotyping. The results of spoligotyping were transferred into a query box in the SITVIT2 database and Run TB-Lineage in the TB Insight website for the identification of spoligo international type (SIT) number and linages of the isolates, respectively. Statistical Product and Service Solutions (SPSS) 20 was applied for statistical analysis. Results Three hundred and fifteen isolates were grouped under 181 different spoligotype patterns. The most dominantly isolated spoligotype pattern was SIT149 and it consisted of 23 isolates. The majority of the isolates were grouped under Euro-American (EA), East-African-Indian (EAI), and Indo-Oceanic (IO) lineages. These lineages consisted of 79.4, 9.8, and 9.8% of the isolates, respectively. One hundred and sixty-five of the isolates were classified under 31 clustered spoligotypes whereas the remaining 150 were singleton types. Furthermore, 91.1% of the total isolates were classified as orphan types. Clustering of spoligotypes was associated ( p < 0.001) with EAI lineage. Conclusion SIT149 and EA lineage were predominantly isolated from the Oromia region substantiating the findings of the similar studies conducted in other regions of Ethiopia. The observation of significant number of singleton and orphan spoligotypes warrants for additional genetic typing of the isolates using method(s) with a better discriminatory power than spoligotyping.

【 授权许可】

CC BY