Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil

Memórias do Instituto Oswaldo Cruz

Silvana Spíndola De Miranda¹  Wânia Da Silva Carvalho¹  Philip Noel Suffys¹  Afrânio Lineu Kritski¹  Martha Oliveira¹  Noemi Zarate¹  Thierry Zozio¹  Nalin Rastogi¹  Brigitte Gicquel² 

[1] ,Universidade Federal de Minas Gerais Faculdade de Medicina Departamento de Clínica Médica/PneumologiaBelo Horizonte MG ,Brasil

关键词: Mycobacterium tuberculosis;    spoligotyping;    Brazil;    Minas Gerais;   

DOI  :  10.1590/S0074-02762011000300003

来源: SciELO

【 摘 要 】

We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region.

【 授权许可】

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