Establishment of Clonal Colony-Forming Assay System for Pancreatic Stem/Progenitor Cells

Cell Transplantation

Article

Hiromitsu Nakauchi¹  Atsushi Suzuki²  Kazunobu Oyama²  Hideki Taniguchi²  Katashi Fukao² 

[1] Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (Japan Science and Technology Corporation), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;Department of Surgery, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;

关键词: Pancreas;    Stem cell;    Colony;    Flow cytometry;   

DOI  :  10.3727/000000002783985765

来源: Sage Journals

【 摘 要 】

Pluripotent stem cells found in a number of organs are usually in small cell populations. However, under adaptive stimulation, they enter the stage of growth and differentiation to compensate for the loss of differentiated cells. To analyze stem cell potential precisely, the exclusion of other differentiated cells and a clonal assay system are strongly required. In this study, we established a colony-forming assay system for pancreatic stem/progenitor cells in vitro. In this culture condition, they received signals for growth and differentiation, and formed clonal colonies including pancreatic endocrine-lineage cells, such as α and β cells. By combining this culture system with flow cytometric cell sorting, pancreatic stem/progenitor cells will be enriched, and their potential can be analyzed precisely in single cell-based experiments.

【 授权许可】

Unknown   
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