Clonal Expansion of Hepatic Stem/Progenitor Cells following Flow Cytometric Cell Sorting

Cell Transplantation

Article

Hiromitsu Nakauchi¹  Atsushi Suzuki²  Yun-Wen Zheng²  Hideki Taniguchi²  Katashi Fukao² 

[1] Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (Japan Science and Technology Corporation), 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;Department of Surgery, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;

关键词: Hepatocyte;    Stem cell;    Flow cytometry;    Integrin;   

DOI  :  10.3727/000000001783986602

来源: Sage Journals

【 摘 要 】

Although hepatic stem cells are believed to exist and play a critical role in developing and regenerating liver, little is known about their cell surface specificity or differentiation capabilities. To make prospective studies of hepatic stem cells possible, we established an in vitro culture system for identification and characterization of hepatic stem/progenitor cells. By combining this culture system with fluorescence activated cell sorting (FACS), a population of cells that were capable of forming large colonies and providing their descendants for relative longer period was isolated from fetal mouse livers. These data suggest that hepatic stem/progenitor cells with high proliferative potential are existent in the developing mouse liver, and that they are enriched by using flow cytometry.

【 授权许可】

Unknown   
© 2001 Cognizant Comm. Corp.