Regulation of cell adhesion signaling by synthetic glycopolymer matrix in primary cultured hepatocyte

FEBS Letters

Kim, Jong-Hun²  Kim, Sang-Heon¹  Akaike, Toshihiro¹ 

[1] Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan;Department of Chemical Engineering, Korean Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-ku, Taejon 305-701, South Korea

关键词: Extracellular matrix;    Cell adhesion;    Integrin;    Asialoglycoprotein receptor;    Glycopolymer;    Hepatocyte;    ASGPR;    asialoglycoprotein receptor;    PVLA;    poly-[N-p-vinylbenzyl-4-O-β-D-galactopyranosyl-D-gluconamide];    PLL;    poly-L-lysine;    ECM;    extracellular matrix;    FN;    fibronectin;    FAK;    focal adhesion kinase;    QCM;    quartz crystal microbalance;    AFM;    atomic force microscopy;    PS;    polystyrene;   

DOI  :  10.1016/S0014-5793(03)01047-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Control of cell–matrix interactions is a central principle for the design of biomaterial in tissue engineering. In this study, we evaluated a synthetic glycopolymer, which is recognized by the asialoglycoprotein receptor (ASGPR) expressed on the surface of hepatocytes, as an artificial matrix to regulate integrin-mediated signaling. The phosphorylation of focal adhesion kinase was restricted in hepatocytes cultured on the glycopolymer compared with fibronectin. In addition, there was no reorganization of cytoskeleton-related proteins such as actin filaments, microtubules, and vinculin in hepatocytes cultured on the glycopolymer. DNA synthesis and cyclin D1 expression were suppressed in hepatocytes grown on the glycopolymer as compared with those grown on fibronectin and collagen. The data suggest that the glycopolymer will be a good artificial matrix to regulate integrin-mediated signaling and cell growth through the unique ASGPR–carbohydrate interaction.

【 授权许可】

Unknown