Short-Term Heart Retention and Distribution of Intramyocardial Delivered Mesenchymal Cells within Necrotic or Intact Myocardium

Cell Transplantation

Article

Fatiha Maskali¹  Pierre-Yves Marie¹  Gilles Karcher¹  Marie-Helene Laurens¹  Laurent Antunes²  Yan Li³  Frederique Groubatch³  Jean-François Stoltz³  Pablo Maureira³  Jean-Pierre Villemot³  Nguyen Tran⁴ 

[1] Department of Nuclear Medicine, INSERM U684, CHU-Nancy, Rue du Morvan, 54500 Vandoeuvre-lès-Nancy, France;Laboratory of Pathology, CHU-Nancy, Rue du Morvan, 54500 Vandoeuvre-lès-Nancy, France;Laboratory of Surgery School, Faculty of Medicine-Nancy, Avenue de la forêt de Haye, BP184, 54505 Vandoeuvre-lès-Nancy Cedex, France;Laboratory of Surgery School, Faculty of Medicine-Nancy, Avenue de la forêt de Haye, BP184, 54505 Vandoeuvre-lès-Nancy Cedex, France;Department of Cell Therapy and Tissue Engineering, UMR7563 CNRS, Faculty of Medicine-Nancy, Avenue de la forêt de Haye, BP184, 54505 Vandoeuvre-lès-Nancy Cedex, France;

关键词: Myocardial infarction;    Cell transplantation;    Mesenchymal cells;    Radioisotopes;    Imaging;   

DOI  :  10.3727/000000006783981918

 received in 2005-07-25, accepted in 2005-10-05,  发布年份 2006

来源: Sage Journals

【 摘 要 】

Cell therapy with bone marrow mesenchymal stem cells (BMSCs) is a new strategy for treating ischemic heart failure, but data concerning the distribution and retention of transplanted cells remain poor. We investigated the short-term myocardial retention of BMSCs when these cells are directly injected within necrotic or intact myocardium. 111Indium-oxine-labeled autologous BMSCs were injected within either 1-month-old infarction (n = 6) or normal myocardium (n = 6) from rats. Serial in vivo pinhole scintigraphy was scheduled during 1 week in order to track the implanted cells. The myocardial retention of BMSCs was definitely higher in myocardial infarction than in normal myocardial area (estimated percent retention at 2 h: 63 ± 3% vs. 25 ± 4%, p < 0.001) and the estimated cardiac retention values were unchanged in both groups along the 7 days of follow-up. On heart sections at day 7, labeled BMSCs were still around the injection site and appeared confined to the scarred tissue corresponding either to the infarct area or to the myocardium damaged by needle insertion. BMSCs have a higher retention when they are injected in necrotic than in normal myocardial areas and these cells appear to stay around the injection site for at least a 7-day period.

【 授权许可】

Unknown   
© 2006 Cognizant Comm. Corp.