The Use of Clinically Approved Small Particles of Iron Oxide (SPIO) for Labeling of Mesenchymal Stem Cells Aggravates Clinical Symptoms in Experimental Autoimmune Encephalomyelitis and Influences Their In Vivo Distribution

Cell Transplantation

Article

Sabine Conrad¹  Joerg Pintaske²  Rainer Kehlbach³  Rüdiger Bantleon³  Jakub Wiskirchen³  Hartwig Wolburg⁴  Miriam Ayturan⁵  Robert Weissert⁵  Richard Schäfer⁶ 

[1] Department of Anatomy, University of Tübingen, Tübingen, Germany;Department of Diagnostic Radiology, Section on Experimental Radiology, University Hospital Tübingen, Tübingen, Germany;Department of Diagnostic Radiology, University Hospital Tübingen, Tübingen, Germany;Department of Pathology, University of Tübingen, Tübingen, Germany;Experimental Neuroimmunology, Department of General Neurology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany;Institute of Clinical and Experimental Transfusion Medicine, University Hospital Tübingen, Tübingen, Germany;

关键词: Experimental autoimmune encephalomyelitis;    Multiple sclerosis;    Mesenchymal stem cells;    Small particles of iron oxide;    Imaging;    Homing;   

DOI  :  10.3727/096368908786576480

 received in 2007-08-29, accepted in 2008-04-01,  发布年份 2008

来源: Sage Journals

【 摘 要 】

Multiple sclerosis (MS) is an inflammatory and demyelinating disease of the central nervous system (CNS). Mesenchymal stem cells (MSC) have been shown to ameliorate symptoms in experimental autoimmune encephalomyelitis (EAE), a model of MS. Using cloned MSC labeled with clinically approved small particles of iron oxide (SPIO) for treatment of EAE we analyzed the tissue localization of transferred cells. Treatment with unlabeled MSC led to disease amelioration compared to controls. In contrast, treatment with SPIO-labeled MSC lead to increase in disease severity. Treatment with SPIO alone did not alter disease course. After transplantation labeled and nonlabeled MSC were detected in the CNS and the liver with significantly more SPIO-labeled cells present in the CNS. Iron deposition was present in the group treated with SPIO-labeled MSC, indicating that in vivo the initially cell surface-bound iron detached from the MSC. These results could be of great importance for imaging of patients in the clinical setting, indicating that in vivo application of SPIO-labeled MSC needs to be performed with caution because the cell-derived exposure of iron can lead to disease aggravation.

【 授权许可】

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© 2008 Cognizant Comm. Corp.