Characteristics of Mouse Adipose Tissue-Derived Stem Cells and Therapeutic Comparisons between Syngeneic and Allogeneic Adipose Tissue-Derived Stem Cell Transplantation in Experimental Autoimmune Thyroiditis

Cell Transplantation

Article

So Young Park¹  Sung Hwa Hong²  Eun Wha Choi³  Eun Ji Yoon⁴  Il Seob Shin⁴  Sung Keun Kang⁴  Jeong Chan Ra⁴ 

[1] Laboratory Animal Research Center, Samsung Biomedical Research Institute, Gangnam-gu, Seoul, Republic of Korea;Laboratory Animal Research Center, Samsung Biomedical Research Institute, Gangnam-gu, Seoul, Republic of Korea;Department of Otorhinolaryngology-Head and Neck Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam-gu, Seoul, Republic of Korea;Laboratory Animal Research Center, Samsung Biomedical Research Institute, Gangnam-gu, Seoul, Republic of Korea;School of Medicine, Sungkyunkwan University, Gangnam-gu, Seoul, Republic of Korea;Stem Cell Research Center, K-STEMCELL, Gasan-dong, Geumcheon-gu, Seoul, Republic of Korea;

关键词: Adipose tissue-derived stem cells;    Autoimmune thyroiditis;    Autoimmune disease;    Anti-thyroglobulin antibody;    Immunophenotype;    Mouse;   

DOI  :  10.3727/096368913X664586

 received in 2011-09-22, accepted in 2013-02-19,  发布年份 2014

来源: Sage Journals

【 摘 要 】

Previously, we found that the intravenous administration of human adipose tissue-derived mesenchymal stem cells was a promising therapeutic option for autoimmune thyroiditis even when the cells were transplanted into a xenogeneic model without an immunosuppressant. Therefore, we explored the comparison between the therapeutic effects of syngeneic and allogeneic adipose tissue-derived stem cells on an experimental autoimmune thyroiditis mouse model. Experimental autoimmune thyroiditis was induced in C57BL/6 mice by immunization with porcine thyroglobulin. Adipose tissue-derived stem cells derived from C57BL/6 mice (syngeneic) or BALB/c mice (allogeneic) or saline as a vehicle control were administered intravenously four times weekly. Blood and tissue samples were collected 1 week after the last transplantation. Adipose tissue-derived stem cells from mice were able to differentiate into multiple lineages in vitro; however, mouse adipose tissue-derived stem cells did not have immunophenotypes identical to those from humans. Syngeneic and allogeneic administrations of adipose tissue-derived stem cells reduced thyroglobulin autoantibodies and the inflammatory immune response, protected against lymphocyte infiltration into the thyroid, and restored the Th1/Th2 balance without any adverse effects. However, different humoral immune responses were observed for infused cells from different stem cell sources. The strongest humoral immune response was induced by xenogeneic transplantation, followed by allogeneic and syngeneic administration, in that order. The stem cells were mostly found in the spleen, not the thyroid. This migration might be because the stem cells primarily function in systemic immune modulation, due to being given prior to disease induction. In this study, we confirmed that there were equal effects of adipose tissue-derived stem cells in treating autoimmune thyroiditis between syngeneic and allogeneic transplantations.

【 授权许可】

Unknown   
© 2014 Cognizant Comm. Corp.

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