Cell Transplantation | |
Cre/loxP-Based Reversible Immortalization of Human Hepatocytes1 | |
Article | |
Toshihisa Matsumura1  Noriaki Tanaka1  Hirofumi Noguchi1  Takamasa Watanabe1  Naoya Kobayashi1  Toshinori Totsugawa1  Toshiyoshi Fujiwara1  Karen A. Westerman2  Philippe Leboulch2  | |
[1] First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan;Harvard-Massachusetts Institute of Technology, Division of Health Sciences and Technology, Cambridge, MA 02139; | |
关键词: Reversible immortalization; Human hepatocytes; Cre/loxP system; Simian virus 40 large T antigen; | |
DOI : 10.3727/000000001783986558 | |
来源: Sage Journals | |
【 摘 要 】
An ideal alternative to the primary human hepatocytes for hepatocyte transplantation would be to use a clonal cell line that grows economically in culture and exhibits the characteristics of differentiated, nontransformed hepatocytes following transplantation. The purpose of the present studies was to establish a reversibly immortalized human hepatocyte cell line. Human hepatocytes were immortalized with a retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40Tag) gene flanked by a pair of loxP recombination targets. One of the resulting clones, NKNT-3, showed morphological characteristics of liver parenchymal cells and expressed the genes of differentiated liver functions. NKNT-3 cells offered unlimited availability. After an adenoviral delivery of Cre recombinase and subsequent differential selection, efficient removal of SV40Tag from NKNT-3 cells was performed. Here we represent that elimination of the retrovirally transferred SV40Tag gene can be excised by adenovirus-mediated site-specific recombination.
【 授权许可】
Unknown
© 2001 Cognizant Comm. Corp.
【 预 览 】
Files | Size | Format | View |
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RO202212205347459ZK.pdf | 126KB | download |
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